Platinum-GP Retroviral Packaging Cell Line, Pantropic
產(chǎn)品名稱： Platinum-GP Retroviral Packaging Cell Line, Pantropic
英文名稱： Platinum-GP Retroviral Packaging Cell Line, Pantropic
產(chǎn)品編號(hào)： RV-103
產(chǎn)品價(jià)格： 詢價(jià)
產(chǎn)品產(chǎn)地： USA
品牌商標(biāo)： cellbiolabs
更新時(shí)間： 2024-12-10T13:18:22
使用范圍：
- 聯(lián)系人 : 李自轉(zhuǎn)
- 地址 : 上海市浦東新區(qū)天雄路166弄一號(hào)樓三層南單元
- 郵編 : 200030
- 所在區(qū)域 : 上海
- 電話 : 139****5640 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : lizizhuan@yeasen.com
- 二維碼 : 點(diǎn)擊查看
Background
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. However,
conventional NIH-3T3 based retroviral packaging cell lines have limited stability and produce low viral
yields, mainly due to poor expression level of the retroviral structure proteins (gag, pol, env) in the
packaging cells.
The Platinum-GP (Plat-GP) Cell Line, a potent retrovirus packaging cell line based on the 293T cell line,
was generated using novel packaging constructs with an EF1α promoter to ensure longer stability and
high-yield retroviral structure protein expression (gag and pol). Plat-GP cells can be kept in good
condition in for at least 4 months in the presence of drug selection, and can produce retroviruses with an
average titer of 1 x 107 infectious units/mL by transient transfection. In addition, replication competent
retroviruses (RCR) are virtually nonexistent because only coding sequences of viral structural genes are
used, avoiding any unnecessary retroviral sequences.