重組人CD27蛋白（Recombinant Human CD27 Protein），GMP級

產品貨號：GMP-TL709

產品描述（Description）

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD7 on lymphocytes and dendritic cells. The CD27-CD7 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

分子別名（Synonym）

 CD27、TNFRSF7、S152、T14、Tp55

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列編碼人CD27（NCBI Reference Sequence: NP_001233.1）表達帶有Fc標簽在C末端

分子量（Molecular Weight）

重組人 CD27 蛋白包含 394 個氨基酸，預測理論分子量為 46.2 kD

純度（Purity）

≥95%采用SDS-PAGE凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 CD27(FC 標簽)蛋白的濃度 1μg/mL(100μL/孔)能夠結合 CD27 Ligand(His 標簽) 的線性范圍是 0.8-15ng/mL

穩定性（Stability）

凍干的樣本的可以在4℃保存24個月，溶解后的液體可以于-20℃保存6-12個月，并且避免反復凍融

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

參考文獻

1、 CD27signalingonchronicmyelogenousleukemiastemcellsactivatesWnttargetgenesandpromotesdiseaseprogression.Christia nSchürch,CarstenRiether,MatthiasS.Matter,AlexandarTzankov,AdrianF.Ochsenbein.JClinInvest.2012Feb1;122(2):624–638.Pu blishedonline2012Jan9.doi:10.1172/JCI45977 

2、 CD27expressiondiscriminatesporcineThelpercellswithfunctionallydistinctproperties.KatharinaReutner,JudithLeitner,Andrea Müllebner,AndreaLadinig,SabineEEssler,JCatharinaDuvigneau,MathiasRitzmann,PeterSteinberger,ArminSaalmüller,Wilhelm GernerVetRes.2013;44(1):18.Publishedonline2013Mar11.doi:10.1186/1297-9716-44-18

  注意：For research and manufacturing use.