The kit mainly includes five tubes of reagents: Enzyme Mix, 5× EZscript All-in-one RT Buffer, NC Buffer, Oligo dT18 (20×) and Random Hexamer (20×). Among them, The Enzyme Mix contains DNase, RNase Inhibitor and reverse transcriptase; The 5× EZscript All-in-one RT Buffer contains buffer, dNTPs, etc; Oligo dT18 (20×) and Random Hexamer (20×) are packaged separately, which is convenient for adding gene-specific primers as needed.

The reverse transcriptase is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. And the multiple site-mutations of reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. The reverse transcription reaction products are recommended for PCR, qPCR and gene cloning.