Anti-53BP1抗體[EPR2172(2)]-抗體-抗體-生物在線
深圳市宇德立生物科技有限公司
Anti-53BP1抗體[EPR2172(2)]

Anti-53BP1抗體[EPR2172(2)]

商家詢價

產品名稱： Anti-53BP1抗體[EPR2172(2)]

英文名稱： 53 BP1 antibody 53BP1 antibody FLJ41424 antibody MGC138366 antibody p202 antibody p53 binding protein 1 antibody p53 BP1 antibody p53-binding protein

產品編號： ab175933

產品價格： null

產品產地： 英國

品牌商標： abcam

更新時間： null

使用范圍：

深圳市宇德立生物科技有限公司
  • 聯系人 :
  • 地址 : 深圳市寶安區西鄉寶民二路賢基大廈4E
  • 郵編 :
  • 所在區域 : 廣東
  • 電話 : 133****4454 點擊查看
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  • 郵箱 : 1484332550@qq.com

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應用

Our?Abpromise guarantee?covers the use of?ab175933?in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

應用 Abreviews 說明
WB ? 1/1000 - 1/5000. Detects a band of approximately 450 kDa (predicted molecular weight: 214 kDa).
IHC-P ? 1/60 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link:?http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).
ICC/IF ? 1/100 - 1/250.

靶標

  • 功能May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • 疾病相關Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • 序列相似性Contains 2 BRCT domains.
  • 翻譯后修飾Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • 細胞定位Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.

  • Anti-53BP1 antibody [EPR2172(2)] 圖像

    • All lanes :?Anti-53BP1 antibody [EPR2172(2)] (ab175933) at 1/1000 dilution (purified)

      Lane 1 :?Mouse heart tissue lysate
      Lane 2 :?Mouse brain tissue lysate

      Lysates/proteins at 10 μg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size :?214 kDa
      Observed band size :?450 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-53BP1 antibody [EPR2172(2)] (ab175933) at 20 μg (purified) + Rat heart tissue lysate at 20 μg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size :?214 kDa
      Observed band size :?450 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes :?Anti-53BP1 antibody [EPR2172(2)] (ab175933) at 1/5000 dilution (purified)

      Lane 1 :?Human fetal heart tissue lysate
      Lane 2 :?HeLa whole cell lysate

      Lysates/proteins at 20 μg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size :?214 kDa
      Observed band size :?450 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling 53BP1 with purified ab175933 at a dilution of 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.?ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling 53BP1 with purified ab175933 at a dilution of 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.?ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling 53BP1 with purified ab175933 at a dilution of 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.?ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.