免疫球蛋白G Fc段受體I
產(chǎn)品名稱： 免疫球蛋白G Fc段受體I
英文名稱： FCGR1A/CD64
產(chǎn)品編號(hào)： 3511
產(chǎn)品價(jià)格： null
產(chǎn)品產(chǎn)地： 上海
品牌商標(biāo)： 雅吉
更新時(shí)間： null
使用范圍： WB ELISA Flow-Cyt
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| 中文名稱 | 免疫球蛋白G Fc段受體I |
| 別????名 | CD 64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ia receptor; F Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for; Fc fragment of IgG, high affinity Ib, receptor (CD64); Fc fragment of IgG, high affinity Ib, receptor for; Fc fragment of IgG, high affinity Ic, receptor (CD64); Fc fragment of IgG, high affinity Ic, receptor for; Fc gamma receptor; Fc gamma receptor I A1; Fc gamma receptor I; Fc gamma receptor I B2; Fc gamma RI; Fc of IgG high affinity Ia receptor; Fc-gamma RIC; FCG 1; FCG1; FcgammaRI; FCGR 1; FCGR1; FCGR1A; FCGR1B; FCGR1C; FcRI; FCRIC; IGFR 1; IGFR1; IGFRB; IGFRC; IgG Fc receptor I; IgG Fc receptor IC; Immunoglobulin G Fc receptor I; Immunoglobulin G Fc receptor IB; Immunoglobulin G Fc receptor IC.?? |
| 研究領(lǐng)域 | 細(xì)胞生物??免疫學(xué)??細(xì)胞膜受體??細(xì)胞表面分子?? |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,?Mouse,? (predicted: Rat,?Pig,?Cow,?) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000?ELISA=1:500-1000?Flow-Cyt=1μg /test? not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 41kDa |
| 細(xì)胞定位 | 細(xì)胞膜? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human CD64/IGFR1:101-200/374? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ)?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The high affinity receptor for immunoglobulin G CD64 is encoded by a family of 3 genes that share over 98% of DNA sequence homology: FCGR1A, FCGR1B and FCGR1C. The FCGR1C gene, like FCGR1A, is located on 1q21. The third gene of this family, FCGR1B, was found at 1p12. CD64 plays a pivotal role in the immune response. It is a glycoprotein (MW 72 kD) that is constitutively expressed on human monocytes and macrophages. Subunit: Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G. Tissue Specificity: Monocyte/macrophage specific. Similarity: Belongs to the immunoglobulin superfamily. FCGR1 family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P12314 Gene ID: 2209 Database links: Entrez Gene: 2209?Human Omim: 146760?Human SwissProt: P12314?Human Unigene: 77424?Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD64是一種糖蛋白，主要定位于單核細(xì)胞和巨噬細(xì)胞,參與免疫應(yīng)答反應(yīng)。 |
| 產(chǎn)品圖片 |  Sample:U937(Human) Cell Lysate at 30 ug Primary: Anti-FCGR1A (bs-3511R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD  Blank control: Mouse spleen cells(blue).Primary Antibody: Rabbit Anti- FCGR1A/CD64 antibody(bs-3511R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol Primary antibody (bs-3511R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.  Blank control: 293T Cells(blue).Primary Antibody: Rabbit Anti-FCGR1A/CD64/AF647 Conjugated antibody (bs-3511R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.  Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).Primary Antibody: Rabbit Anti-CD64 antibody(bs-3511R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
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