EFM-192B（人乳腺癌細胞）
產品名稱： EFM-192B（人乳腺癌細胞）
英文名稱： EFM-192B
產品編號： RE59712
產品價格： 0
產品產地： 中國
品牌商標： 雅吉生物
更新時間： null
使用范圍： null
上海雅吉生物科技有限公司
- 聯系人 :
- 地址 : 上海市閔行區元江路5500號第1幢5658室
- 郵編 :
- 所在區域 : 上海
- 電話 : 158****3937 點擊查看
- 傳真 : 點擊查看
- 郵箱 : yajikit@163.com
?
Synonyms: EFM-192B Background: established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; cells were described in the literature to overexpress the erB-2 gene; sister cell lines EFM-192A and -192C show differences in molecular aspects Species: human (Homo sapiens) Tissue: breast?? Disease: breast carcinoma Gender: N/A? Morphology: epitheloid adherent cells growing as monolayers Growth Mode: N/A? Doubling Time: ca. 4-5 days DNA Profile: N/A
?Cell Line Authentication ServiceCulture Medium: PMI-1640+20%FBS
We strongly suggest to purchase the complete medium from .Cryopreservation medium: 90%FBS+10%DMSO Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 -, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Fingerprint: same DNA profile as EFM-192A/EFM-192C using multiplex PCR of minisatellite markers Species: confirmed as human with IEF of LDH, MDH, NP Cytogenetics: human heteroploid hypotriploid karyotype with 13% polyploidy - 62(58-62)<3n>XXX/XX, -X, +2, +4, +6, -7, -8, -8, -10, -11, -12, +14, +16, -17, -17, -17, -18, -18, -19, -20, -21, -22, +8mar, add(1)(p22-31), add(2)(q?35), del(3)(p22), add(5)(p15), del(6)(q25), add(10)(p11), add(13)(q32), add(14)(p12), add(15)(p12), der(15)t(10;15)(q11;p12), der(20)t(1;20)(q24;q12/13), der(21)t(7;21)(q11;p12)x2 - most cells carry a small ring and 1-2 large submetacentric 15p+ markers - closely related to EFM-192A - apparent ch 17 nullisomy Virus?? ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - ?
? ? ? ?