Product: Anti-Fas, human, FITC-labeled, Clone APO-1-1
Cat. No: MC-100 (100 tests)
Background:
Monoclonal antibody recognizing Fas protein,
FITC-labeled for simplified flow cytometry
procedures. Clone Apo-1-1 is an IgG1 isotype
switch variant of the IgG3 clone APO-1-3 (Cat.
No. MC-095).
Specificity:
Reacts specifically with the human Fas antigen,
although the epitope has not been mapped.
Binds both the soluble and membrane-bound
forms of Fas. Does not react with mouse or
gibbon Fas. Recognizes activated T-cells and a
subpopulation of B-cells.
The cell-surface Fas antigen is a 48 kDa
transmembrane protein that can mediate
apoptosis/programmed cell death and belongs
to the nerve growth factor (NGF)/tumor necrosis
factor (TNF) receptor superfamily. The human
Fas antigen is expressed in various human cells,
including activated peripheral T and B
lymphocytes, Various tumor cell lines, leukemic
T cells, and myeloid cells. It is also differentially
expressed on human thymocytes during thymic
maturation.
Ig Isotype: IgG1
Immunogen:
Human B lymphoblast cell line SKW6.4 cell
surface molecules
Hybridoma:
Mouse myeloma (P3.X63.Ag8.653) x immunized
mouse (Balb/c) spleen cells.
Format:
FITC-labeled antibody in 1 ml PBS, pH 7.4 with
1% BSA and 0.02% NaN3. Purified by protein A
chromatography to >95% by SDS-PAGE.
Storage and Stability:
Store unopened vial at 4°C. After opening,
aliquot and store at –20°C.
Applications and Suggested Dilutions:

Immunohistochemistry on cryostat sections
and on cytospin preparations.1 Not
suitable for use on paraffin sections.

Flow Cytometry: Use at 1:20 dilution (as
determined on SKW6.4 cells.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.