Product: Anti-APRIL, clone Sacha-2
Cat. No.: MC-092 (100 μg)
Synonyms:
A-Proliferation-inducing Ligand, CD256, TNFSF
13.
Specificity:
Recognizes human and mouse APRIL.
Species Reactivity:
Human and mouse. Others species not tested.
Ig Isotype:
Rat IgG2a
Immunogen:
Recombinant human APRIL (aa 105-250) fused
to human ACRP30 headless (aa 16-108).
Format:
100 μL of a 1 mg/mL solution of antibody
purified from concentrated hybridoma tissue
culture supernatant. Antibody is in PBS with
0.02% sodium azide. >95% purity as determined
by SDS-PAGE.
Storage:
Store at 4°C.
Applications:

ELISA (capture)

Flow cytometry

Immunocytochemistry
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.
Figure 1: Immunocytochemistry on a frozen section of HEK-
293T cells transfected with a human APRIL expression
plasmid (left) or mock transfected (right).
Method: HEK-293T cells expressing surface membrane
associated human APRIL (aa 92-233) or a mock control
were injected in chicken liver and frozen in OCT. 8 μm
sections of injected frozen liver were fixed in acetone,
washed in PBS and incubated with anti-APRIL (MC-092) at
10 μg/mL for 1 hour after blocking in PBS/1% BSA. Human
APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC)
following 45 minutes incubation with goat anti-rat HRP.
Figure 2: Antibody MC-092 detects membrane-bound human
APRIL by FACS.
Method: HEK 293T cells (5 x 105) were mock transfected
(thin line) or transfected with an expression plasmid enabling
surface expression of mouse APRIL (thick line). Cells were
incubated on ice for 30 min in 50 μL FACS buffer (PBS, 5%
Fetal calf serum, 0.02% sodium azide) containing 1 μg/mL of
anti-APRIL (MC-092). After washing in FACS buffer, PEconjugated
antibody to rat IgG was added. Cells were
incubated on ice for 30 minutes, washed and then analyzed
by flow cytometry.