Anti-FBXW7 (R479L) Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-FBXW7 (R479L) Mouse Monoclonal Antibody

Anti-FBXW7 (R479L) Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-FBXW7 (R479L) Mouse Monoclonal Antibody

英文名稱： Anti-FBXW7 (R479L) Mouse Monoclonal Antibody

產品編號： 26347

產品價格： null

產品產地： 美國

品牌商標： NewEastbio

更新時間： null

使用范圍： ELISA, WB, IHC

武漢紐斯特生物技術有限公司
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FBXW7 (R479L) [26347]
[100ul]		 ?

Catalog Number:??26347
Gene Symbol:??FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10
Description: ??Anti-FBXW7 (R479L) Mouse Monoclonal Antibody
Background: ??F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.
Immunogen: ??A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin.
Applications: ??ELISA, WB
Recommended Dilutions: ??
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes R479L mutant, but not wild-type FBXW7 of vertebrates.
Storage Conditions:?? Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant FBXW7 (R479L) and wildtype proteins. Purified His-tagged FBXW7 (R479L) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-FBXW7 (R479L) monoclonal antibody (Cat. #26347).
Immunofluorescence:??

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Immunofluorescence of cells expressing FBXW7 proteins with anti-FBXW7 (R479L) antibody. HEK293T cells were transfected with pCDNA3-GFP-FBXW7 (WT) plasmid (left column) or pCDNA3-GFP-FBXW7 (R479L) plasmid (right column), then fixed and stained with anti-FBXW7 (R479L) monoclonal antibody (Cat. #26347).

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