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Description

Dickkopf-related protein 1 (DKK-1) is a protein that in humans is encoded by the DKK1 gene, which is mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopfFamily. It is a secreted protein with two cysteine-rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Elevated levels of DKK-1 in bone marrow, plasma, and peripheral blood are associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

ScienCells human DKK-1 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human DKK-1-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human DKK-1in the sample that is captured on the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice

Reference

1. "Entrez Gene: DKK1 dickkopf homolog 1 (Xenopus laevis)"
2. Roessler, E., Du, Y., Glinka, A., Dutra, A., Niehrs, C., Muenke, M. The genomic structure, chromosome location, and analysis of the human DKK1 head inducer gene as a candidateFor holoprosencephaly. Cytogenet. Cell Genet. 89: 220-224, 2000.?

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???????近幾年的新藥研發(fā)成功率在逐年下降，其根本原因之一就是傳統(tǒng)的藥物篩選系統(tǒng)是建立在只具有30-40%人類基因群的一系列細胞株上。這樣一個有“缺陷型”藥物篩選系統(tǒng)所產(chǎn)生的藥物用于人體上就會出現(xiàn)許多致命的弱點和不完整性。新一代藥物篩選系統(tǒng)是含有一系列近乎完整的人類基因群的原代細胞株。而利用這些原代細胞株所甄別和篩選出來的候選藥物，其診治人類疾病的成功幾率將大大增加。這樣不但大大節(jié)省了新藥的開發(fā)成本，而且將極大地提高人類的健康質量。這些產(chǎn)品從根本上提高了全球生命醫(yī)學研究、人類重要疾病藥物研發(fā)、新藥研發(fā)的成功率。所以上海中喬新舟生物科技有限公司致力于提供優(yōu)質原代細胞產(chǎn)品和完善的售后服務。?
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???????美國ScienCell研究實驗室（www.sciencellonline.com）成立于1999年，公司總部位于美國加州的圣地亞哥。主要致力于實驗室科研用原代細胞、原代細胞專用培養(yǎng)基、原代細胞無血清培養(yǎng)基、干細胞、干細胞培養(yǎng)基、干細胞無血清培養(yǎng)基的研究和開發(fā)，在全球擁有眾多客戶。在國內(nèi)銷售15年來，很多老師應用其產(chǎn)品發(fā)表了高質量的SCI文章，憑借著嚴格的質控和優(yōu)秀的產(chǎn)品品質，深受廣大科研工作者的信賴。

????????ScienCell研究實驗室生產(chǎn)的原代細胞、原代細胞專用培養(yǎng)基都經(jīng)過了嚴格的質量控制，細胞純度可達98％。其中包括21種人體正常細胞系統(tǒng)，90多種不同細胞類型。大多數(shù)細胞在全球唯有ScienCell實驗室能夠成功分離，產(chǎn)品質量過硬。?確保了實驗結果的真實性、重復性和連貫性。

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