Size??100ug
Cat code??200767
Species Cross-reactivity??H,Mk
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??25kDa
Sensitivity??This antibody detects endogenous levels of UCHL1 and does not cross-react with related proteins.
Immunogen??Purified recombinant human UCHL1 protein fragments expressed in E.coli.
UniProt Number??P09936
GeneBank ID??NM_004181.4
Subcellular location??Cytoplasm
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Altername??Neuron cytoplasmic protein 9.5；PGP 9.5；Ubiquitin thioesterase L1
Gene_symbol??PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
Summary??The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
UniPort summary  Function??Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated
proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of
ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have
ATP-independent ubiquitin ligase activity
Application Images :??
Western blot detection of UCHL1 in U251,IMR32,Raji&COS7 cell lysates and  using UCHL1 antibody (1:1000 diluted). 
Predicted band size: 25KDa 
Observed band size: 28KDa
Immunocytochemistry stain of COS7 using UCHL1 antibody (1:300).