Product: Anti-c-Met (Hepatocyte Growth Factor Receptor) mAb, clone 8F11
Cat. No.: MC-140 (1 mL)
Background:
The c-Met proto-oncogene encodes a
transmembrane tyrosine kinase identified as the
receptor for a polypeptide known as hepatocyte
growth factor (HGF). HGF has been shown to
exert a pleiotropic activity on several cell types
mainly of epithelial origin. It is a powerful mitogen
for hepatocytes and also stimulates the growth of
other cell types including kidney tubular cells,
keratinocytes and endothelial cells. Its receptor,
c-Met, has been identified in 45 percent of
prostatic carcinoma and in 75 percent of
metastatic growth of prostatic carcinoma. In
melanocytic neoplasm, c-Met has been detected
in 6 percent of primary melanoma and in 39
percent of metastatic lesions. c-Met is expressed
at late stages of melanoma progression and
suggests that its presence may contribute to the
acquisition of an invasive phenotype. Other cell
types known to express c-Met include
hepatocytes, microglial cells in white matter and
astrocytes.
Specificity:
Recognizes the external domain of the beta
chain of human c-Met.
Species Reactivity:
Human. Others not tested.
Ig Isotype:
Mouse IgM
Immunogen:
Recombinant protein corresponding to the
external domain of the beta chain of the c-Met
molecule.
.
Format:
Lyophilized tissue culture supernatant containing
15 mM sodium azide. Reconstitute with 1 mL of
sterile distilled water as indicated on vial label.
Storage and Stability:
Store unopened lyophilized antibody at 4°C.
Under these conditions, there is no significant
loss in product performance up to the expiration
date indicated on the vial label. The reconstituted
antibody is stable for at least two months at 4°C.
For long term storage, aliquot and store at -20°C
Avoid freeze-thaw cycles. Prepare working
dilutions on the day of use.
Positive Control:
Prostate epithelium.
Staining Pattern:
Membrane
Applications and Suggested Dilutions:

Immunohistochemistry (frozen and paraffin):
Typical working dilution 1:20 – 1:30. High
temperature antigen unmasking technique. 60
minutes primary antibody incubation at 25°C.
Standard ABC technique.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.