Size??100ug
Cat code??220001
Species Cross-reactivity??H
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??
Isotype??IgG1
Molecular Weight??100kDa
Sensitivity??This antibody detects endogenous levels of MSH2 and does not cross-react with related proteins.
Immunogen??Purified recombinant fragment of human MSH2 expressed in E. Coli.
UniProt Number??P43246
GeneBank ID??NM_000251
Subcellular location??Nucleus
Formulation??Ascitic fluid containing 0.03% sodium azide.
Altername??BAT26;COCA 1;COCA1;DNA mismatch repair protein Msh2;FCC 1;FCC1;hMSH2;HNPCC 1;HNPCC;HNPCC1;LCFS2;MSH 2;Msh2;MSH2_HUMAN;MutS homolog 2;MutS homolog 2 colon cancer nonpolyposis type 1;MutS protein homolog 2.
Gene_symbol??FCC1;COCA1;HNPCC;LCFS2;HNPCC1.
Summary??This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).When cloned,it was discovered to be a human homolog of the E. coli mismatch repair gene mutS,consistent with the characteristic alterations in microsatellite sequences (RER  phenotype) found in HNPCC.Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq,Apr 2012]
UniPort summary  Function??Component of the post-replicative DNA mismatch repair system (MMR).Forms two different heterodimers:MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair.When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs.MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long.After mismatch binding,MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer,which is thought to be responsible for directing the downstream MMR events,including strand discrimination,excision,and resynthesis.ATP binding and hydrolysis play a pivotal role in mismatch repair functions.The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch:mismatched DNA provokes ADP-->ATP exchange,resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone.This transition is crucial for mismatch repair.MutS alpha may also play a role in DNA homologous recombination repair.In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Application Images :??
Western blot detection of MSH2 antibody in Hela,A431,A549 and 293T cell lysates using MSH2 antibody (1:500 diluted).Predicted band size:100KDa.Observed band size:100KDa.
Immunocytochemistry staining of HeLa cells using anti-MSH2 antibody (dilution 1:100).