Breast Cancer Biomarker組合(Ki67， ER alpha， ErbB2， p53， PgR)-抗體-抗體-生物在線
深圳市宇德立生物科技有限公司
Breast Cancer Biomarker組合(Ki67， ER alpha， ErbB2， p53， PgR)

Breast Cancer Biomarker組合(Ki67， ER alpha， ErbB2， p53， PgR)

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產品名稱： Breast Cancer Biomarker組合(Ki67， ER alpha， ErbB2， p53， PgR)

英文名稱： Breast Cancer Biomarker組合(Ki67， ER alpha， ErbB2， p53， PgR)

產品編號： ab104827

產品價格： null

產品產地： 英國

品牌商標： abcam

更新時間： null

使用范圍：

深圳市宇德立生物科技有限公司
  • 聯系人 :
  • 地址 : 深圳市寶安區西鄉寶民二路賢基大廈4E
  • 郵編 :
  • 所在區域 : 廣東
  • 電話 : 133****4454 點擊查看
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  • 郵箱 : 1484332550@qq.com

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  • ab104827 is a human breast cancer biomarker panel containing 50μg of Ki67 rabbit polyclonal, 50μg of estrogen receptor alpha rabbit polyclonal, 50μg of ErbB 2 rabbit polyclonal, 50μg of p53 mouse monoclonal and 50μg of progesterone receptor mouse monoclonal antibodies. This panel contains a selection of biomarkers used to identify breast cancer cells. Much interest has been focused on cancer biomarkers, as recent studies have shown that they are useful for predicting cancer disease progression.
    The components of this product are:
    ab15580 Rabbit polyclonal to Ki67 - Proliferation Marker?50μg Concentration: Lot specific Applications: Flow Cyt: 1/100 ICC/IF: 1/100 - 1/1000 IHC-P: 1/200 IHC-Fr: 1/100-1000 IHC-FoFr: Use at an assay dependent dilution IHC-FrFl: 1/50 WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 345, 395 kDa (predicted molecular weight: 359 kDa)
    ab37438 Rabbit polyclonal to Estrogen Receptor alpha?50μg Concentration:1 mg/ml Applications: ICC/IF: Use at a concentration of 5 μg/ml IHC-P: Use at a dilution of 1/100 WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 68 kDa
    ab2428 Rabbit polyclonal to ErbB 2?250μl Concentration: 0.2 mg/ml Applications: IHC-P: Use at an assay dependent dilution IHC-Fr: Use at an assay dependent dilution IP: Use at an assay dependent dilution WB: 1/200
    ab26 Mouse monoclonal [PAb 240] to p53?50μg Concentration: Lot specific Applications: ELISA: 1/100 Flow Cyt: Use at an assay dependent dilution ICC/IF: Use at an assay dependent dilution IHC-P: 1/250 - 1/500 IHC-Fr: 1/250 - 1/500 IHC (Methanol fixed): 1/250 IP: Use at 10μg/mg of lysate WB: 1/1000 - 1/2000. Detects a band of approximately 53 kDa
    ab2764 Mouse monoclonal [PR-AT 4.14] to Progesterone Receptor - ChIP Grade50μg Concentration:1 mg/ml Applications: ChIP: Use at an assay dependent dilution. (PubMed: 19553525) ICC/IF: 1/500. Using T47D cells. IHC-P: Use at a concentration of 5 μg/ml. IHC-Fr: Use at a concentration of 5 μg/ml. Immunohistochemical staining of PR in rat uterus with this antibody yields intense nuclear staining. IP: Use at an assay dependent dilution. WB: Use at a concentration of 5 μg/ml. By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from rat uterine homogenate. Predicted molecular weight: 99 kDa. (PubMed: 16912069)
    Storage Instructions?Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • 組件

Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) 圖像

  • Paraffin embedded HER2+ tumor (IDC31) sections incubated with ErbB2 (ab2428). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/100 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 5 min. (This antibody has also been tested successfully on xenograft derived from ER+ pleural effusion and the HER2+ amplified breast cancer cell line BT474).
  • Paraffin embedded primary ER+ breast tumor sections incubated with Progesterone Receptor (ab2764). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/100 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 5-10 min.
  • Paraffin embedded primary ER+ breast tumor sections incubated with Estrogen receptor alpha (ab37438). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/50 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 5 min. (This antibody has also been tested successfully on xenograft derived from ER+ pleural effusion).
  • Paraffin embedded primary ER- breast tumor sections incubated with p53 (ab26). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/50 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 10 min.?