山羊Anti-Rabbit IgG H&L (HRP) preadsorbed
產(chǎn)品名稱： 山羊Anti-Rabbit IgG H&L (HRP) preadsorbed
英文名稱： 山羊Anti-Rabbit IgG H&L (HRP) preadsorbed
產(chǎn)品編號： ab97080
產(chǎn)品價格： null
產(chǎn)品產(chǎn)地： 英國
品牌商標(biāo)： abcam
更新時間： null
使用范圍：
- 聯(lián)系人 :
- 地址 : 深圳市寶安區(qū)西鄉(xiāng)寶民二路賢基大廈4E
- 郵編 :
- 所在區(qū)域 : 廣東
- 電話 : 133****4454 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : 1484332550@qq.com
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- 靶標(biāo)種屬Rabbit
- 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
- 經(jīng)測試應(yīng)用ICC,?IHC-P,?ELISA,?WBmore details
- 最小交叉反應(yīng)
Chicken, Cow, Horse, Human, Mouse, Pig, Ratmore details - 偶聯(lián)物HRP
性能
- 形式Liquid
- 存放說明Shipped at 4°C. Store at +4°C.
- 存儲溶液Preservative: 0.1% Proclin
Constituents: 0.2% BSA, PBS -
濃度500 μg 濃度為 0.5 mg/ml?
- 純度Immunogen affinity purified
- 純化說明Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
- 克隆多克隆
- 同種型IgG
- 研究領(lǐng)域
- Secondary antibodies ?
- anti-Rabbit ?
- IgG ?
- Enzyme ?
- HRP
應(yīng)用
Our?Abpromise guarantee?covers the use of?ab97080?in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed 圖像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with?ab8227, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97080,?1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080)Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution developed using the ECL technique, with?ab87342.