Size??100ug
Cat code??300195
Species Cross-reactivity??
Key Application??WB
Host??Rabbit
Antibody type??Polyclonal antibody
Purified method??Affinity purified
Isotype??
Molecular Weight??26kDa
Sensitivity??This antibody detects endogenous levels of GST and does not cross-react with related proteins.
Immunogen??Purified recombinant Escherichia coli BL21(DE3) GST protein fragments expressed in E.coli.
UniProt Number??
GeneBank ID??YP_002999397
Subcellular location??Protein sequence and structure are important classification methods for GSTs:while classes from the cytosolic superfamily of GSTs possess more than 40% sequence homology,those from other classes may have less than 25%. Cytosolic GSTs are divided into 13 classes based upon their structure:alpha,beta,delta,epsilon,zeta,theta,mu,nu,pi,sigma,tau,phi,and omega.Mitochondrial GSTs are in class kappa.The MAPEG superfamily of microsomal GSTs consists of subgroups designated I-IV, between which amino acid sequences share less than 20% identity.The glutathione binding site, or "G-site," is located in the thioredoxin-like domain of both cytosolic and mitochondrial GSTs.The region containing the greatest amount of variability between the assorted classes is that of helix α2,where one of three different amino acid residues interacts with the glycine residue of glutathione.Two subgroups of cytosolic GSTs have been characterized based upon their interaction with glutathione:the Y-GST group,which uses a tyrosine residue to activate glutathione,and the S/C-GST,which instead use serine or cysteine residues.The porcine pi-class enzyme pGTSP1-1 was the first GST to have its structure determined,and it is representative of other members of the cytosolic GST superfamily,which contain a thioredoxin-like N-terminus domain as well as a C-terminus domain consisting of alpha helices.Mammalian cytosolic GSTs are dimeric, both subunits being from the same class of GSTs,although not necessarily identical.The monomers are in the range of 2230 kDa.They are active over a wide variety of substrates with considerable overlap.
Formulation??Purified Rabbit polyclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername??Glutathione S Transferase;Glutathione S transferase Mu 1;Glutathione S-transferase class-mu 26 kDa isozyme;GST 26;GST;GST class mu 1;GST1;GSTM1 1;GSTM1a 1a;GSTM1b 1b;GTH4;GTM1;H B;HB subunit 4;MGC26563;MU 1;MU;SJ26 antigen;SjGST.
Gene_symbol??
Summary??The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial,and microsomal (now designated as MAPEG) proteins.GSTs are present in eukaryotes and in prokaryotes,where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates.Members of the GST superfamily are extremely diverse in amino acid sequence,and a large fraction of the sequences deposited in public databases are of unknown function.The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.GSTs can constitute up to 10% of cytosolic protein in some mammalian organs.GSTs catalyse the conjugation of reduced glutathione  via a sulfhydryl group  to electrophilic centers on a wide variety of substrates in order to make the compounds more soluble.This activity detoxifies endogenous compounds such as peroxidised lipids, as well as breakdown of xenobiotics.GSTs may also bind toxins and function as transport proteins,which gave rise to the early term for GSTs of “ligandin”.The mammalian GST super-family consists of cytosolic dimeric isoenzymes of 4555 kDa size that have been assigned to at least six classes:Alpha,Mu,Pi,Theta,Zeta and Omega.Most mammalian isoenzymes have affinity for the substrate 1-chloro-2,4-dinitrobenzene (CDNB),and spectrophotometric assays utilising this substrate are commonly used to report GST activity.However,some endogenous compounds,e.g., bilirubin,can inhibit the activity of GSTs.In mammals,GST isoforms have cell specific distributions (e.g., alpha GST in hepatocytes and pi GST in the biliary tract of the human liver).
UniPort summary  Function??null
Application Images :??
Western blot detection of GST antibody in CHO-K1 transfected with GST cell lysate and GST recombinant protein fragments，purified recombinant human GST-Lin28B & GST-Lin28A protein fragments expressed in E.coli,using GST antibody (1:10000 diluted).