• 描述
  兔單克隆抗體[EPR5873(2)] to 14-3-3 sigma
• 經測試應用WB,?IHC-Pmore details
• 種屬反應性
  與反應:?Rat, Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 14-3-3 sigma aa 50-150. The exact sequence is proprietary.
  Database link:?P31947

• 陽性對照
  • Human skin, A431 and HACAT lysates; Human 14-3-3 Sigma recombinant protein; Human tonsil tissue.
• 常規說明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^??technology. RabMAb^??technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存儲溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
• 濃度100 μl 濃度為 1.258 mg/ml?40 μl 濃度為 1.258 mg/ml?10 μl 濃度為 1.258 mg/ml?
  
• 純度Protein A purified
• 克隆單克隆
• 克隆編號EPR5873(2)
• 同種型IgG
• 研究領域
  • Neuroscience
  ?
  • Neurology process
  ?
  • Neural Signal Transduction

  • Signal Transduction
  ?
  • Adapters
  ?
  • Cytoplasmic

  • Neuroscience
  ?
  • Cell Type Marker
  ?
  • Neuron marker
  ?
  • Synapse marker

  • Stem Cells
  ?
  • Signaling Pathways
  ?
  • Wnt
  ?
  • 14-3-3

應用

靶標

• 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
  p53-regulated inhibitor of G2/M progression.
• 組織特異性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
• 序列相似性Belongs to the 14-3-3 family.
• 翻譯后修飾Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
• 細胞定位Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
• Information by UniProt
• 數據庫鏈接
  • Entrez Gene: 2810?Human
  • Entrez Gene: 313017?Rat
  • Omim: 601290?Human
  • SwissProt: P31947?Human
  • Unigene: 523718?Human
• 別名
  • 14 3 3 protein sigma antibody
  • 14-3-3 protein sigma antibody
  • 1433S_HUMAN antibody

  see all

Anti-14-3-3 sigma antibody [EPR5873(2)] 圖像

• 

  Western blot - Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667)
  All lanes :?Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667) at 1/1000 dilution
  
  Lane 1 :?Human skin lysate
  Lane 2 :?A431 cell lysate
  Lane 3 :?HACAT cell lysate
  
  Lysates/proteins at 20 μg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size :?27 kDa
  Observed band size :?28 kDa (why is the actual band size different from the predicted?)
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Western blot - Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667)
  Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667) at 1/1000 dilution + Human 14-3-3 Sigma recombinant protein with His tag at 10 μg
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size :?27 kDa
  Observed band size :?28 kDa (why is the actual band size different from the predicted?)
  

  Human full length 14-3-3 sigma recombinant protein is a house-made one with His-Tag.

  Blocking/Dilution buffer: 5% NFDM/TBST.

• TUNEology 波長可調檢測卡盒-->