• 形式Liquid
• 存放說明Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C long term. Avoid freeze / thaw cycle.
• 存儲溶液pH: 7.4
  Preservative: 0.1% Proclin
  Constituents: PBS, Sodium chloride, Sodium phosphate, 30% Glycerol, 1% BSA
• 濃度250 μl 濃度為 1 mg/ml?
  
• 純度Purified via His tag
• 純化說明This product is a recombinant protein produced in E. coli.
• 克隆單克隆
• 研究領域
  • Secondary antibodies
  ?
  • anti-Rabbit
  ?
  • IgG
  ?
  • Enzyme
  ?
  • HRP

應用

Anti-Rabbit IgG VHH Single Domain Antibody (HRP) 圖像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rabbit IgG sdAb (ab191866)

  IHC image of?Anti-Rabbit IgG VHH Single Domain Antibody (HRP)?(ab191866) staining in formalin fixed paraffin embedded normal human colon tissue section.

  The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit polyclonal antibody to beta tubulin?(ab6046, 0.5μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody,?Anti-Rabbit IgG VHH Single Domain Antibody (HRP)?(ab191866, 0.125μg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

  The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Western blot - sdAb anti-Rabbit IgG HRP (ab191866)
  All lanes :?Anti-NRG1 type III antibody (ab23248) at 1 μg/ml
  
  Lane 1 :?Mouse Brain Tissue Lysate
  Lane 2 :?Rat Brain Tissue Lysate
  Lane 3 :?Mouse Brain Tissue Lysate
  Lane 4 :?Rat Brain Tissue Lysate
  
  Lysates/proteins at 10 μg per lane.
  
  Secondary
  Lanes 1 - 2 :?Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 μg/ml
  Lanes 3 - 4 :?Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) at 0.05 μg/ml
  
  developed using the ECL technique
  
  Performed under reducing conditions.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - sdAb anti-Rabbit IgG HRP (ab191866)

  IHC image of Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human?cerebellum tissue section.

  The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit monoclonal antibody [EPR12763] to NeuN (ab177487, 0.1μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody,?Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 1.0μg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

  The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - sdAb anti-Rabbit IgG HRP (ab191866)

  Top left: IHC image of Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human colon tissue section.

  The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit polyclonal antibody to Ki67 (ab15580, 0.1μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody,?Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 0.1μg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

  Top right: this image shares the same experimental design parameters, except the secondary antibody wasab97051, goat anti-rabbit IgG H&L (HRP) (0.1μg/ml). This demonstrates the improved definition of staining given by VHH Single Domain Antibodies over conventional secondaries.

  Bottom right: this image shares the same experimental design parameters but is a negative control (no primary antibody) for?ab97051, demonstrating specificity of the goat anti-rabbit secondary antibody.

  Bottom left: this image shares the same experimental design parameters but is a negative control (no primary antibody) for ab191866, demonstrating the specificity of the VHH-Single Domain Antibody.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rabbit IgG sdAb (ab191866)

  IHC image of Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human colon tissue section.

  The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit polyclonal antibody to Ki67 (ab15580, 0.1μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP)?(ab191866, 0.025μg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

  The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rabbit IgG sdAb (ab191866)

  IHC image of Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human colon tissue section.

  The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with rabbit polyclonal antibody to VDAC1 (ab15895, 1/1000 dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody,?Anti-Rabbit IgG VHH Single Domain Antibody (HRP)?(ab191866, 0.125μg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

  The negative control (secondary antibody only, no primary) insert shows no staining, demonstrating secondary antibody specificity.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Western blot - sdAb anti-Rabbit IgG HRP (ab191866)
  All lanes :?Anti-GAPDH antibody (ab37168) at 1 μg/ml
  
  Lane 1 :?HeLa Whole Cell Lysate
  Lane 2 :?Jurkat Whole Cell Lysate
  Lane 3 :?HeLa Whole Cell Lysate
  Lane 4 :?Jurkat Whole Cell Lysate
  
  Lysates/proteins at 10 μg per lane.
  
  Secondary
  Lanes 1 - 2 :?Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.02 μg/ml
  Lanes 3 - 4 :?Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) at 0.02 μg/ml
  
  developed using the ECL technique
  
  Performed under reducing conditions.