重組人SIRP α蛋白（Recombinant Human SIRPα Protein），GMP級

產品貨號：GMP-TL729

產品描述（Description）

Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells。

分子別名（Synonym）

SHPS1、SIRPA、CD172A、BIT、MFR、MYD1、P84、PTPNS1

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列編碼人SIRP alpha（NCBI Reference Sequence:NP_542970.1）表達帶有His標簽在C末端

分子量（Molecular Weight）

重組人SIRP alpha蛋白 包含241個氨基酸，預測理論分子量為39kD

純度（Purity）

≥95%采用 SDS-PAGE 凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 CD47(Fc 標簽)5ug/mL(100 ul/孔)能夠結合人 SIRPα(His 標簽)的線性范圍是 10-500 ng/mL

穩定性（Stability）

凍干的樣本的可以在4℃保存24個月，溶解后的液體可以于-20℃保存6-12個月，并且避免反復凍融

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

產品關鍵詞：重組人SIRPα蛋白、SIRPα;

參考文獻

1. Signal regulatory protein alpha (SIRPα) regulates the homeostasis of CD103+CD11b+ DCs in the intestinal lamina propria Charlotte L Scott, Zangerle Murray TFP, Katherine S H Beckham, Gillian Douce, Allan McI Mowat Eur J Immunol. 2014 Dec; 44(12): 3658–3668. Published online 2014 Oct 27. doi: 10.1002/eji.201444859

  注意：For research and manufacturing use.