Disease:

Esophageal carcinoma 

Origin:

Established from the adenocarcinoma of the lower oesophagus (Barrett's metaplasia), equivalent to JROECL33 

Species:

Homo sapiens 

Tissue:

Esophagus 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

Express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice 

Patient:

Female, Caucasian, 73 years of age 

Medium:

RPMI1640 + 10% FBS 

Subculture:

1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Protein Expression:
 cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - 
Mutations in OE33 Cells 

Related cells by Organ:

EC-109 cells 

HKESC-1 cells 

OE33 cells 

JROECL33 cells 

NE-1 cells 


References for OE33 cells:

1. Zhang, Q., Zhao, X. H., and Wang, Z. J. Flavones and flavonols exert cytotoxic effects on a human oesophageal adenocarcinoma cell line (OE33) by causing G2/M arrest and inducing apoptosis. Food Chem Toxicol, 46: 2042-2053, 2008. 

2. Beales, I. L. and Ogunwobi, O. O. Leptin synergistically enhances the anti-apoptotic and growth-promoting effects of acid in OE33 oesophageal adenocarcinoma cells in culture. Mol Cell Endocrinol, 274: 60-68, 2007. 

3. Cheong, E., Ivory, K., Doleman, J., Parker, M. L., Rhodes, M., and Johnson, I. T. Synthetic and naturally occurring COX-2 inhibitors suppress proliferation in a human oesophageal adenocarcinoma cell line (OE33) by inducing apoptosis and cell cycle arrest. Carcinogenesis, 25: 1945-1952, 2004.