SPECIFICATION?SHEET

Catalog?#:????L1C01102????????????????Lot?#:????C070301
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Description:?????????MAb?to?CA724?,?Monoclonal?Antibody?to?Human?CA724
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Specificity:????The?CA724?is?a?tumor?associated?antigen?with?a?molecular?weight?of?>1000Kd.?LS-174T??carcinoma?cells.
Clone:????CC49

Host?Animal:????Mouse.??Hybridization?of?P3-X63-Ag8.653?.myeloma?cells?with?spleen?cells?from?Balb/c?mice.????

Isotype:?????IgG2a,?
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Source:?????mouse?Ascites

Immunogen:???????BALB/C?mice?were?injected?with?membrane?enriched?fraction?of?a?human?LS-174T??carcinoma?cells.?

Format:????Purified,?Liquid

Purification:??????>90%?pure?(SDS-PAGE).??Protein?A?chromatography.

Concentration:?????2mg/ml??(0.7×OD280nm)

Affinity?Constant:??????1?x?10-10?mol/l.

Buffer:????10mM?Sodium?Phosphate,?0.15M?Sodium?Chloride,?pH?7.4

Preservative:?????none

Applications:?????Immunohistochemistry?on?frozen?or?paraffin?sections.??Each?laboratory?should?determine?an?optimum?working?titer?for?use?in?its?particular?application.?
Recommended?antibody?pair?for?immunoassay:
????Capture????Detection
????L1C01101????????????????L1C01102
Storage:????Upon?receipt,?aliquot?and?store?at?<–40°C.??Avoid?multiple?freeze/thaw?cycles.??If?aliquoted?for?long?term?storage,?fill?volume?should?be?equal?to?or?greater?than?50%?of?the?nominal?fill?volume?of?the?vial?used.