別名（Synonyms）

Wingless-type MMTV Integration Site Family, Member 5a; wingless-type MMTV integration site family, member 5A; Wnt5a

表達系統（Source）

HEK293 Cells

表達序列（Sequence）

Gln38-Lys380

Accession

P41221

分子量大?。∕olecular Weight）

The protein has a predicted MW of 40kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation

純度（Purity）

> 90% by SDS-PAGE.

內毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

生物活性（Biological Activity）

Measured by its ability to inhibit Wnt-3a-induced a TCF/LEF luciferase reporter

expression by 293F cells. The ED50 for this effect is 2-10µg/mL. Optimal concentrations

should be determined by each laboratory for each application

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and 0.01 % Tween 80 were added as protectant before lyophilization

標簽（Tag）

His tag at the C-terminal

外觀（Physical Appearance）

凍干粉

復溶（Reconstitution）

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.