Lipofectamine 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

 Superior performanceour highest efficiency reagent for difficult-to-transfect cells.
 Improved cell viabilitygentle on your cells, with low toxicity.
 Versatileone reagent for DNA, RNA, and co-transfection.

Efficiently Transfect Difficult-to-Transfect Cells:
Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7). Myoblast (C2C12, L6 CRL-1458).
Hepatocyte (HepG2, HuH7.) Erythroleukemic cell (K562).
Breast carcinoma (MCF7, Hs578T). Prostate carcinoma (LNCap).
Lung carcinoma (A549, NCI-H460). Osteosarcoma (U-2 OS, Saos-2).
Colon carcinoma (Caco2, SW480). Pancreatic carcinoma (PANC-1).
Skin melanoma (SK-MEL-28).  


Transfect Common Cells Efficiently and Cost-Effectively:
For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity:
Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 L for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications:
Lipofectamine 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.