The plasmid DNA is rapidly bound onto a column with a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The ZymoPURE  Express Plasmid Midiprep kit’s fast and simple protocol enables the recovery of highly concentrated transfection-grade plasmid DNA in just 15 minutes. As an added convenience, the ZymoPURE  Express Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.                                            HIGHLIGHTS：
Direct lysis protocol omits conventional cell-pelleting and resuspension steps.
Purify up to 1.2 mg of highly concentrated plasmid DNA directly from a spin-column.
Prepare transfection-grade plasmid DNA in only 15 minutes.