PIK3CA(E545G)點突變單克隆抗體-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
PIK3CA(E545G)點突變單克隆抗體

PIK3CA(E545G)點突變單克隆抗體

商家詢價

產品名稱： PIK3CA(E545G)點突變單克隆抗體

英文名稱： Anti PIK3CA(E545G) Mouse Monoclonal Antibody

產品編號： 26208

產品價格： null

產品產地： 美國

品牌商標： NewEastbio

更新時間： null

使用范圍： ELISA, WB, IHC

武漢紐斯特生物技術有限公司
  • 聯系人 :
  • 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
  • 郵編 : 430074
  • 所在區域 : 湖北
  • 電話 : 186****7297 點擊查看
  • 傳真 : 點擊查看
  • 郵箱 : neweast3@126.com

?

PIK3CA (E545G) [26208]
[100ul]		 ?

Catalog Number:??26208
Gene Symbol:??PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
Description: ??Anti-PIK3CA (E545G) Mouse Monoclonal Antibody
Background: ??Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
Immunogen: ??A synthetic peptide from the internal region of PIK3CA which includes the mutation of E545G, human origin.
Applications: ??ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes E545G mutant, but not wild-type PIK3CA of vertebrates.
Storage Conditions:?? Store at -20°C. Avoid freeze / thaw cycles
?
Western blot:??
Western blot analysis of recombinant PIK3CA (E545G) and wildtype proteins. Purified His-tagged PIK3CA (E545G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-PIK3CA (E545G) monoclonal antibody (Cat. #26208).
Immunofluorescence:??
Immunofluorescence of cells expressing PIK3CA proteins with anti-PIK3CA (E545G) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA (E545G) plasmid (right column), then fixed and stained with anti-PIK3CA (E545G) monoclonal antibody (Cat. #26208).

?

?