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FormofAntibody:MouseIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.
Storage/Stability:Storeat-20℃/1year
Clonality:Monoclonal
Immunogen:TheantiserumwasproducedagainstasyntheticpeptideDYKDDDDKcoupledtoKLH.
Purification:Theantibodywasaffinity-purifiedfromrabbitantiserumbyaffinity-chromatographyusingepitope-specificimmunogen.
Specificity/Sensitivity:Flag-TagMouseMonoclonalantibodydetectsendogenouslevelsofC-terminal,internal,andN-terminalFlag-taggedproteins.
Reactivity:All
Applications:WB:1:5000IP:1:200IF:1:1000
Theoptimalworkingdilutionshouldbedeterminedbytheenduser.
References:

WB:

All lanes : Flag-Tag Mouse Monoclonal antibody A: HEK 293 cell lysate without transfected B: HEK 293 cell lysate transfected with FLAG-tag protein C: IP (PBS + anti- FLAG mAb 4℃ overnight) D: IP (HEK293 transfected+protein G+anti- FLAG mAb 4℃ overnight) E: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) F: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain

IHC:N/A

IF:

Immunofluorescence analysis of 293T cells transfected with a FLAG-tagged protein, using Flag-Tag Mouse Monoclonal antibody at a 1:2000 dilution (blue DAPI, red anti-FLAG).