description:for research or for further manufacturing use.
sterility;sterile-filtered.
form;liquid.
storage condition;protect from light.
technique(s);cell culture | mammalian: suitable.
pH;7.1-7.7.
components;L-glutamine: no;HEPES: 7.5 mM;NaHCO3: 1.60 g/L;phenol red: no;glucose: 3.42 g/L.
Application;EX-CELL 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or the GS System, because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.