Product Highlight

• Configuration-specific monoclonal antibody that recognizes Arl3-GTP, but not Arl3-GDP
• The only available Arl3 activation assay kit directly measures the level of Arl3-GTP
• Providing shorter experimental time
• Could be used to monitor the activation of Arl3 in cells and in tissues by immunohistochemistry

Product Description

Arl3 (Arf-like 3) is an ADP-ribosylation factors (Arf) family protein. Arl3 interacts with and regulates activities of several effector proteins, such as human retinal gene 4 (HRG4), δ-subunit of the cGMP phosphodiesterase (PDEδ), and binder of Arl2 (BART). Arl3 also binds microtubules to alter specific aspects of cytokinesis via interactions with retinitis pigmentosa 2 (RP2).

Currently there is no direct assay to measure the activation of Arl3 GTPases.

NewEast Biosciences Arl3 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Arl3-GTP, but not Arl3-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

These anti-Arl3-GTP monoclonal antibodies can also be used to monitor the activation of Arl3 in cells and in tissues by immunohistochemistry.

NewEast Biosciences Arl3 Activation Assay Kit provides a simple and fast method to monitor the activation of Arl3. Each kit provides sufficient quantities to perform 20 assays.

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Assay Principle

NewEast Biosciences Arl3 Activation Assay Kit bases on the configuration-specific anti-Arl3-GTP monoclonal antibody to measure the active Arl3-GTP levels, either from cell extracts or from in vitro GTPγS loading Arl3 activation assays. Briefly, anti-active Arl3 mouse monoclonal antibody will be incubated with cell lysates containing Arl3-GTP. The bound active Arl3 will then be pulled down by protein A/G agarose. The precipitated active Arl3 will be detected by immunoblot analysis using anti- Arl3 rabbit polyclonal antibody.