Product: Anti-Bax pAb
Cat. No.: PC-518 (100 μg)
Synonyms:
Anti-Bcl-2 Associated Protein X.
Background:
Apoptosis plays a major role in normal organism
development, tissue homeostasis, and removal
of damaged cells. Disruption of this process has
been implicated in a variety of diseases such as
cancer. The Bcl-2 family of proteins is comprised
of critical regulators of apoptosis that can be
divided into two classes: those that inhibit
apoptosis and those that promote cell death.
Bax, a pro-apoptotic Bcl-2 family member, is a
cytosolic protein that changes conformation and
translocates to the mitochondria following
apoptotic stimuli. It is thought to share significant
functional homology with Bak, another proapoptotic
Bcl-2 family member, as disruption of
Bax or Bak has little effect on cell death, but
mice lacking both genes display multiple
developmental defects and cells lacking both
show decreased apoptotic capability.
Specificity:
Recognizes Bax.
Species Reactivity:
Human. Other species not tested.
Host:
Rabbit
Immunogen:
A 16 amino acid peptide from near the aminoterminus
of human Bax (GenBank Accession No.
AAA03619).
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by ion exchange
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 2 μg/mL.

Immunocytochemistry: Use at ~2 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of Bax in HL-60 cell
lysates with anti-Bax pAb at (A) 1 μg/mL, (B) 2 μg/mL,
and (C) 4 μg/mL.
Figure 2: Immunocytochemistry of Bax in HL-60 cells
with anti-Bax pAb at 2 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product