Anti-Active Rap1 Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-Active Rap1 Mouse Monoclonal Antibody

Anti-Active Rap1 Mouse Monoclonal Antibody

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產品名稱： Anti-Active Rap1 Mouse Monoclonal Antibody

英文名稱： Anti-Active Rap1 Mouse Monoclonal Antibody

產品編號： 26912

產品價格： null

產品產地： 美國

品牌商標： NewEastbio

更新時間： 2023-09-21T11:43:01

使用范圍： IP, IHC

武漢紐斯特生物技術有限公司
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  • 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
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  • 所在區域 : 湖北
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Active Rap1-GTP [26912]
[30 μL]		 ?

Catalog Number:??26912
Gene Symbol:??RAP1
Description: ??Anti-Active Rap1 Mouse Monoclonal Antibody
Background: ??Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins.
Immunogen: ??Recombinant full length protein of active Rap1.
Applications: ??IP, IHC
Recommended Dilutions: ??1 μg for 1~2 mg total cellular proteins for IP
1:100 dilution for IHC
Concentration: ??1 mg/ml
Host: ??mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??IgG
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Anti-active Rap1 antibody recognizes active Rap1 of vertebrates.
Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles
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Immunoprecipitation/Western blot:??
Rap1 activation assay. Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rap1 monoclonal antibody (Cat. No. 26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. 21104).
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Publications:
1.??Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo
????PLoS One. 2013 Sep 10;8(9):e73070

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