Recommended for use with the RNAqueous? Kit for purification of total RNA from plant tissues. One bottle

of 10 mL Ambion? reagent is provided.
? Removes common plant contaminants such as polyphenolics and polysaccharides
? No phenol extraction, alcohol precipitation, or proteinase digestion steps required when used with the

?RNAqueous? kit Ambions Plant RNA Isolation Aid contains polyvinylpyrrolidone (PVP), a high molecular weight

?polymer that binds to contaminants such as polyphenolics and polysaccharides that are commonly present in

plant tissues. Glass fiber filter–based methods of isolation, such as the RNAqueous? Kit, are especially

suited for plant RNA isolation because it does not include any alcohol precipitation steps; many common

contaminants found in plant tissue are known to co-precipitate with nucleic acid during alcohol precipitation.

?When used with the RNAqueous? Kit, the procedure consists of disrupting the plant tissue in a

guanidinium-based lysis solution to which the Plant RNA Isolation Aid has been added. A brief centrifugation

then removes the contaminants. The supernatant is passed through a glass fiber filter which binds RNA. The

filter is then washed and the RNA is eluted in a small volume (50 μL or more) of elution solution.

Accessory Product: The Plant RNA Isolation Aid is designed for use with the RNAqueous? Kit (SKU #AM1912) for

?RNA purification from plant tissues