軸突生長因子CD108抗體
產品名稱： 軸突生長因子CD108抗體
英文名稱： CD108
產品編號： 2702
產品價格： null
產品產地： 上海
品牌商標： 雅吉
更新時間： null
使用范圍： WB ELISA IHC-P IHC-F IF
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| 中文名稱 | 軸突生長因子CD108抗體 |
| 別????名 | semaphorin 7A; CD 108; CD108; CD108 antigen; CDw108; H Sema K1; H Sema L; JMH; JMH blood group antigen; John Milton Hagen blood group H Sema K1; John Milton Hargen human blood group Ag; MGC126692; MGC126696; Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A; Sema L; Sema7a; SEMAK1; SEMAL; Semaphorin 7A GPI membrane anchor; Semaphorin K1; Semaphorin L; Semaphorin7a; SEM7A_HUMAN.?? |
| 研究領域 | 細胞生物??免疫學??生長因子和激素??轉錄調節因子?? |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,?Mouse,?Rat,? (predicted: Dog,?Cow,?) |
| 產品應用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?ICC=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 66kDa |
| 細胞定位 | 細胞膜?細胞外基質? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human Sema7A:351-450/666? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | Semaphorin 7a is required for central and peripheral axon growth and is required for proper axon tract formation during embryonic development. Semaphorin 7a is also involved in modulating immune function. Semaphorin 7a expression is highest in the brain and increases in late embryonic and postnatal stages. The Semaphorins constitute a large family of secreted and membrane-tethered cell signaling molecules. They have functions in neural development, immunology, cardiac growth, vascular development, lung morphogenesis and axial bone patterning. Semaphorins are defined by the presence of a conserved Sema domain at the N-terminus. These proteins can be classified into eight classes depending on their structure and species origin. Classes 3 through 7 are found in vertebrates; class 3 are secreted, class 7 are GPI-anchored, and class 4 through 6 are transmembrane proteins. Function: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. Subunit: Interacts with ITGA1 and ITGB1 (Probable). Interacts with PLXNC1. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Note=Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. Tissue Specificity: Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. Similarity: Belongs to the semaphorin family. SWISS: O75326 Gene ID: 8482 Database links: Entrez Gene: 8482?Human Entrez Gene: 20361?Mouse Entrez Gene: 315711?Rat Omim: 607961?Human SwissProt: O75326?Human SwissProt: Q9QUR8?Mouse Unigene: 24640?Human Unigene: 335187?Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產品圖片 |  Sample:Skin (Mouse) Lysate at 40 ug Skin(Rat) Lysate at 40 ug Primary: Anti-CD108 (bs-2702R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 66 kD  Sample:Cerebrum (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-CD108 (bs-2702R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 63 kD  Sample:Lane1: Brain (Mouse) Lysate at 30 ug Lane2: Lung (Mouse) Lysate at 30 ug Primary: Anti-Sema7A/CD108(bs-2702R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 66kD Observed band size : 66kD  Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD108) Polyclonal Antibody, Unconjugated (bs-2702R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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