28-4055-00
            
GST MULTITRAP 4B
        
        

            
28-4055-01
            
GST MULTITRAP FF
        
        

            
28-9523-59
            
GST SpinTrap
        
        

            
28-9523-60
            
GST GraviTrap
        
        

            
28-9523-61
            
GST Buffer Kit
        
        

            
28-9545-49
            
PGEX-4T-1
        
        

            
28-9545-50
            
PGEX-4T-2
        
        

            
28-9545-52
            
PGEX-4T-3
        
        

            
28-9545-53
            
PGEX-5X-1
        
        

            
28-9545-54
            
PGEX-5X-2
        
        

            
28-9545-55
            
PGEX-5X-3
        
        

            
28-9546-46
            
PGEX-2TK
        
        

            
28-9546-48
            
PGEX-6P-1 VECTOR
        
        

            
28-9546-50
            
PGEX-6P-2 VECTOR
        
        

            
28-9546-51
            
PGEX-6P-3 VECTOR
        
        

            
28-9546-53
            
PGEX-2T 25 UG WI/DI
        
        

            
28-9546-54
            
PGEX-3X 25 UG WI/DI
        
        

            
28-9546-56
            
PGEX-1LT ECOR I/BAP
        
        

            
11-0033-99
            
HIS GRAVITRAP
        
        

            
11-0034-00
            
HIS BUFFER KIT
        
        

            
27-4710-01
            
ANTI-HIS ANTIBODY
        
        

            
28-4009-89
            
HIS MULTITRAP HP
        
        

            
28-4009-90
            
HIS MULTITRAP FF
        
        

            
28-4013-53
            
HIS SPINTRAP
        
        

            
28-4039-43
            
COLLECTION PLATE
        
        

            
28-9321-71
            
His SpinTrap Kit
        
        

            
27-1583-01
            
LIGHT PRIMER MIX
        
        

            
27-1586-01
            
HEAVY PRIMER MIX
        
        

            
27-1588-01
            
LINKER-PRIMER MIX
        
        

            
27-1589-01
            
RS PRIMER MIX
        
        

            
27-9420-01
            
ANTI-M13 MONOCLONAL ANTIBODY
        
        

            
27-9421-01
            
HRP/ANTI-M13 MONOCLNL CONJUG.
        
    




詳情請查詢以下網址：
http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/zh/GELifeSciences-us/28952360

GST GraviTrap
GST GraviTrap contains 10 disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein

    
Convenient, prepacked gravity-flow columns.
    
Allows direct purification of both clarified and unclarified cell lysates.
    
Yields up to 20 mg of pure proteins per column.
    
Yields up to 20 mg of pure proteins per column

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GST GraviTrap contains 10 disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein. GST-tagged proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the proteins.
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The binding capacity of prepacked GST GraviTrap columns, which can be greater than 10 mg protein/ml medium, varies with the nature of the tagged protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged proteins.
GST GraviTrap — Technical Specifications


    

        

            
Complete Packsize
            
10 x 2 ml
        
        

            
Bed Volume
            
2 ml
        
        

            
Binding Capacity/Column
            
Up to 20 mg recombinant glutathione S-transferase (rGST)
        
        

            
Storage Conditions
            
4 to 30°C, 20% Ethanol
        
    
    



    

        

            
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Media
            
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Ligand
            
Glutathione
        
        

            
Average Particle Size
            
90 μm
        
        

            
Matrix
            
Agarose, 4%
        
        

            
Particle Size
            
45 μm-165 μm
        
        

            
Binding Capacity/ml Chromatography Medium
            
> 25 mg horse liver GST/ml medium1)
        
        

            
pH stability Working Range
            
4-13
        
        

            
pH stability Cleaning
            
4-13
        
        

            
Storage Conditions
            
4 to 30°C, 20% Ethanol
        
        

            
Chemical Stability
            
All commonly used aqueous buffers
        
    
    

    

        
1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
        
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Column
            
?
        
        

            
Column i.d.
            
15 mm
        
        

            
Chemical Stability
            
Stable in all commonly used buffer systems
        
        

            
Material [Column Hardware]
            
Polypropylene (PP)
        
        

            
Material [Bed Support]
            
Polyethylene (PE)