GLUTATHIONE SEPH 4 FF-色譜介質-耗材-生物在線
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GLUTATHIONE SEPH 4 FF

GLUTATHIONE SEPH 4 FF

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產品名稱： GLUTATHIONE SEPH 4 FF

英文名稱： GLUTATHIONE SEPH 4 FF

產品編號： 17-5132-02

產品價格： 0

產品產地： 美國

品牌商標： GE

更新時間： null

使用范圍： null

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17-5132-02 GLUTATHIONE SEPH 4 FF, 100 ML
17-5132-03 GLUTATHIONE SEPH 4 FF, 500 ML
17-5138-01 RMP PROT A SEPH FF 5 ML
17-5138-02 RMP PROT A SEPH FF 25 ML
17-5138-03 RMP PROT A SEP FF 200 ML
17-5199-01 MABSELECT 25 ML
17-5199-02 MABSELECT 200 ML
17-5268-01 NI SEPHAROSE HP 25 ML
17-5268-02 NI SEPHAROSE HP 100 ML
17-5269-02 MABSELECT XTRA 200 ML
17-5269-07 MABSELECT XTRA 25ML
17-5279-01 GLUTATHIONE SEPHAROSE HP 25 ML
17-5279-02 GLUTATHIONE SEPH HP 100 ML
17-5280-01 NPROTEIN A SEPHAROSE 4 FF 5ML
17-5280-02 NPROTEIN A SEPH 4 FF 200 ML
17-5280-04 NPROTEIN A SEPHAROSE 4FF 25ML
17-5318-01 NI SEPHAROSE 6FF, 25 ML
17-5318-02 NI SEPHAROSE 6FF, 100 ML
17-5318-03 NI SEPHAROSE 6FF, 500 ML
17-5318-06 NI SEPHAROSE 6 FF 5 ML
17-5438-01 MABSELECT SURE 25 ML
17-5438-02 MABSELECT SURE 200 ML
17-5448-01 Capto Blue 25 ml
17-5474-01 MabSelect SuRe LX 25 ML
17-5474-02 MabSelect SuRe LX 200 ML
17-6002-35 IMMUNOPRECIP STARTER PACK
18-1022-29 HB AFFINITY CHROMATOGRAPHY
18-1037-46 ANTIBODY PURIFICATION HB
28-9355-97 Dextrin Sepharose High Perform
28-9355-98 Dextrin Sepharose High Perform
28-9356-00 StrepTactin Sepharose High Per
28-9805-82 MembraneProteinPurificationKit
17-5468-15 HiScreen Capto SP ImpRes
17-5470-15 HiScreen Capto Q ImpRes
17-5474-15 HiScreen MabSelect SuRe LX

Glutathione Sepharose 4 Fast Flow - Scale-up Purification

  • Fast, one-step purification of GST*-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins.
  • Glutathione coupled to Sepharose? 4 Fast Flow via a?10-carbon spacer arm.
  • Excellent for scale-up due to high capacity and high flow properties.

Technical Information


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Glutathone Sepharose 6 Fast Flow for purification of GST-tagged proteins.

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Glutathione Sepharose? 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other?S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose? 4 Fast Flow provides good flow properties and is excellent for scaling up.

For more information of the full range of products included in the Glutathione S-transferase (GST*) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System. A more comprehensive guide to the GST Gene Fusion System is available in handbook 18-1157-58, see Media Reference Books, Software and Video Cassettes.

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TECHNICAL SPECIFICATIONS
Ligand? Glutathione?
Ligand coupling method? Epoxy activation?
Matrix? Highly cross-linked agarose, 4%?
Average particle size? 90 μm?
Binding capacity*? ? 10 mg recombinant GST/ml medium?
pH stability? 3-12?
Chemical stability? All commonly used aqueous buffers,?e.g. 1?M acetate, pH?6.0, 0.1?M NaOH, ?70% ethanol, 6?M guanidine hydrochloride?(1?h at room temperature)?
Storage? 20% ethanol?
Storage temperature? 4°C to 30°C?
*Binding of GST to glutathione is flow-rate dependent and lower flow rates often
increase the binding capacity. This is important to consider during sample
loading and elution.