| Catalog Number:??10120 |
| Product Name:??Rap1B Protein |
| Synonyms:??Member of RAS oncogene family, K-REV, RAL1B |
| Source: ??Human, recombinant full length, His6-tag |
| Expression Host: ??E. coli |
| Molecular Weight: ??21 kDa |
| Purity: ??>95% by SDS-PAGE |
| Introduction: ??Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway. |
| Amino Acid Sequence ??(1-184) |
MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAM?
RDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNL?
ARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL |
Properties
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| Physical Appearance (form): ??Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. |
| Physical Appearance (form):??White or clear |
| Concentration:??1 mg/mL |
| Storage:???-80°C |
Preparation Instructions:??
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rap1B was determined by SDS- PAGE and Coomassie Brilliant Blue Staining |
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References:??
hrzanowska-Wodnicka, M. et al., J. Clin. Invest. 115: 680-687, 2005.?
2. Hoshijima, M. et al., Biochem. Biophys. Res. Commun. 157: 851-860, 1988.?
3. Kawata, M. et al., Proc. Nat. Acad. Sci. 87: 8960-8964, 1990.?
4. Kawata, M. et al., J. Biol. Chem. 264: 15688-15695, 1989.?
5. Lova, P. et al., J. Biol. Chem. 278: 131-138, 2003.?
6. Matsui, Y. et al., Biochem. Biophys. Res. Commun. 166: 1010-1016, 1990.?
7. Pizon, V. et al., Nucleic Acids Res. 16: 7719-only, 1988.?
8. Rehmann, H. et al., Nature 455: 124-127, 2008.?
9. Rousseau-Merck, M. F. et al., Cytogenet. Cell Genet. 53: 2-4, 1990.?
10. Schwamborn, J. C. et al., Nature Neurosci. 7: 923-929, 2004. |
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