人結腸癌細胞(HCT116)-細胞株/菌種-試劑-生物在線

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蘇州海星生物科技有限公司
人結腸癌細胞(HCT116)

人結腸癌細胞(HCT116)

商家詢價

產品名稱: 人結腸癌細胞(HCT116)

英文名稱: HCT116

產品編號: TCH-C185

產品價格: 0

產品產地: 蘇州

品牌商標: HyCyte™

更新時間: null

使用范圍: null

蘇州海星生物科技有限公司
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  • 地址 : 太倉市開發區
  • 郵編 : 215300
  • 所在區域 : 江蘇
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  • 郵箱 : hfx@dingbio.com

細胞名稱:人結腸癌細胞(STR鑒定正確)

細胞別稱:HCT-116; HCT.116; HCT_116; HCT116; CoCL2




HCT116是1979年BrattainM等從患結腸癌的男性病人中分離建立的3株惡性細胞之一;產生CEA、角蛋白。在半固體瓊脂糖培養基中形成克隆。HCT116在無胸腺的裸鼠有致瘤性,形成上皮樣的腫瘤。

細胞名稱:人結腸癌細胞

細胞簡稱HCT116

產品貨號TCH-C185

種屬來源

組織來源結腸

疾病特征結腸癌

細胞形態上皮細胞樣

生長特性貼壁生長

培養體系?McCoys 5A+10%FBS+1%P/S

傳代比例:1:3-1:8,每2-3天換液一次

傳代周期48-72 h

培養條件氣相:95%空氣+5%CO2,溫度:37℃

凍存條件60%基礎培養基+30%FBS+10%DMSO,液氮儲存

質量檢測細菌、真菌、支原體檢測均為陰性

參考文獻:

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Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479

Brattain MG, et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756

Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064

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Wang R, et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887"