LDH Cytotoxicity Assay Kit 乳酸脫氫酶(LDH)細胞毒性檢測試劑盒
產品名稱： LDH Cytotoxicity Assay Kit 乳酸脫氫酶(LDH)細胞毒性檢測試劑盒
英文名稱： LDH Cytotoxicity Assay Kit 乳酸脫氫酶(LDH)細胞毒性檢測試劑盒
產品編號： 40209ES
產品價格： 詢價
產品產地： Yeasen
品牌商標： Yeasen
更新時間： 2025-08-01T09:18:38
使用范圍： null
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乳酸脫氫酶細胞毒性檢測試劑盒(LDH Cytotoxicity Assay Kit)，也稱乳酸脫氫酶檢測試劑盒(LDH Assay Kit)或乳酸脫氫酶釋放檢測試劑盒(LDH Release Assay Kit)，是一種基于diaphorase催化的INT顯色反應，通過比色法檢測細胞毒性時釋放的乳酸脫氫酶活性或檢測其它樣品中的乳酸脫氫酶活性的試劑盒。細胞凋亡或壞死而造成的細胞膜結構的破壞會導致細胞漿內的酶釋放到培養液里，其中包括酶活性較為穩定的乳酸脫氫酶(lactate dehydrogenase, LDH)。通過檢測從質膜破裂的細胞中釋放到培養液中的LDH的活性，就可以實現對細胞毒性的定量分析。LDH釋放被看做細胞膜完整性的重要指標，并被廣泛用于細胞毒性檢測。LDH釋放被認為是以前使用放射性的51Cr標記細胞，隨后通過51Cr釋放進行細胞膜完整性檢測的安全有效的替代方法。本試劑盒的基本原理是，在乳酸脫氫酶的作用下，NAD+被還原生成NADH，NADH和INT (2-p-iodophenyl-3-nitrophenyl tetrazolium chloride)被硫辛酰胺脫氫酶(diaphorase)催化反應生成NAD+和強生色物甲臜(formazan)，在490 nm波長下產生吸收峰，從而通過比色來定量檢測細胞培養液、細胞裂解液等樣品中乳酸脫氫酶的活性。吸光度與乳酸脫氫酶活性成線性正相關。該酶聯反應原理的示意圖如下:
即用、便捷。
-25~-15℃保存，避光保存，有效期1年。
Q:血清會影響檢測結果嗎？
A:由于血清含有乳酸脫氫酶，建議血清的使用濃度不要超過 1%，并最好使用熱滅活血清。
Q:收取細胞上清液中釋放的 LDH，在冰箱放置過夜，第二天檢測可以嗎？
A:收取細胞上清液中釋放的 LDH，可以在冰箱放置過夜，第二天檢測。但是為了減少實驗誤差，建議客戶最好是現取現測。
Q: 乳酸脫氫酶細胞毒性檢測試劑盒，對照的死亡率怎么算？
A: 這個試劑盒一般是計算樣品的死亡率, 不計算對照的死亡率呀，而且樣品的死亡率也要用對照的來計算。
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