?新的PathScan? 化學發光夾心ELISA試劑盒比傳統的顯色檢測系統具有更寬的動態范圍和更強的靈敏性.這些試劑盒提供小體積的微孔板，可以在使用小體積樣本時保持強信號和靈敏性。

實驗室的研發，驗證和優化保證了ELISA試劑盒的高質量。

化學發光檢測系統提供了最寬的動態范圍和最好的檢測靈敏度。

技術支持由開發和生產試劑盒的相同科學家提供。

12116????? PathScan? Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit

12321???? PathScan? Phospho-Ret (panTyr) Chemiluminescent Sandwich ELISA Kit

7021?????? PathScan? Phospho-FLT3 (Tyr591) Chemiluminescent Sandwich ELISA Kit

7024?????? PathScan? Phospho-TrkA (Tyr490) Chemiluminescent Sandwich ELISA Kit

7027?????? PathScan? Phospho-c-Jun (Ser63) Chemiluminescent Sandwich ELISA Kit

7036?????? PathScan? Phospho-EGF Receptor (Tyr1068) Chemiluminescent Sandwich ELISA Kit

7084?????? PathScan? Total ALK Chemiluminescent Sandwich ELISA Kit

7087?????? PathScan? Phospho-TrkB (panTyr) Chemiluminescent Sandwich ELISA Kit

7132?????? PathScan? Total Akt1 Chemiluminescent Sandwich ELISA Kit

7134?????? PathScan? Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit

7135?????? PathScan? Phospho-Akt (Thr308) Chemiluminescent Sandwich ELISA Kit

7149?????? PathScan? Phospho-Stat3 (Tyr705) Chemiluminescent Sandwich ELISA Kit

7153?????? PathScan? Phospho-p70 S6 Kinase (Thr389) Chemiluminescent Sandwich ELISA Kit

7297?????? PathScan? Total EGF Receptor Chemiluminescent Sandwich ELISA Kit

7316?????? PathScan? Phospho-S6 Ribosomal Protein (Ser235/236) Chemiluminescent Sandwich ELISA Kit

7337?????? PathScan? Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit

7792?????? PathScan? Phospho-AMPKα (Thr172) Chemiluminescent Sandwich ELISA Kit

7849?????? PathScan? Phospho-SAPK/JNK (Thr183/Tyr185) Chemiluminescent Sandwich ELISA Kit

7869?????? PathScan? Total SAPK/JNK Chemiluminescent Sandwich ELISA Kit

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8026?????? PathScan? Phospho-Stat3 (Ser727) Chemiluminescent Sandwich ELISA Kit

Specificity / Sensitivity

PathScan? Total EGF Receptor Chemiluminescent Sandwich ELISA Kit #7297 detects endogenous levels of EGF receptor protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

PathScan? Total EGF Receptor Chemiluminescent Sandwich ELISA Kit #7297能夠檢測內源性水平的EGF受體蛋白。本試劑盒用來檢測指示物種的蛋白，即通過內部測試確定的那些，也可以用來檢測來自其他物種的同源蛋白。

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Description

PathScan? Total EGF Receptor Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total EGF receptor protein. Chemiluminescent ELISAs often have a wider dynamic range and higher sensitivity than conventional chromogenic detection. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using a smaller sample size. An EGF Receptor Mouse mAb has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-EGF receptor proteins are captured by the coated antibody. Following extensive washing, EGF Receptor Rabbit mAb is added to detect both the captured phospho- and nonphospho-EGF receptor protein. Anti-rabbit IgG, HRP-linked Antibody #7074 is then used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of total EGF receptor protein. ? Antibodies in kit are custom formulations specific to kit.

PathScan? Total EGF Receptor Chemiluminescent Sandwich ELISA Kit采用固相夾心酶聯免疫吸附試驗（ELISA），用化學發光法檢測內源性水平的總表皮生長因子受體蛋白。化學發光酶聯免疫吸附比傳統的顯色檢測往往有更廣闊的動態范圍和更高的靈敏度。此化學發光ELISA提供在低量微孔板，提高信號靈敏度，同時使用一個較小的樣本量。微孔包被EGF受體小鼠單克隆抗體。與細胞裂解物孵育后，磷酸化和非磷酸化-EGF受體蛋白質由包被的抗體捕獲。充分洗滌后，加入EGF受體兔單克隆抗體，以檢測捕獲得磷酸和非磷酸-EGF受體蛋白。然后，使用抗兔IgG，HRP-標記的抗體＃7074，識別結合的檢測抗體。加入化學發光試劑進行顯色。發射光強度用相對光單位（RLU）測量，并與總EGF受體蛋白的量成正比。試劑盒中的抗體為試劑盒特別訂制配方。

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ELISA

Figure 1. The relationship between protein concentration of lysates from A-431 cells, untreated or treated with hEGF #8916, and immediate light generation with chemiluminescent substrate. After starvation, A-431 cells (85% confluence) were treated with hEGF (100 ng/ml, 5 min at 37°C) and then lysed. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.圖1. A-431細胞裂解物的蛋白濃度之間的關系，細胞不處理或用hEGF #8916處理，立即產生光化學發光底物。饑餓處理后，A-431細胞（85%匯合）用hEGF處理 (100 ng/ml, 5 min at 37°C)，然后裂解。圖中對應的陰影部分的面積圖形顯示了高靈敏度和低蛋白質濃度范圍內的線性響應。

ELISA

Figure 2. The relationship between protein concentration of lysates prepared using H1975 cells, lysed with (phospho) and without (nonphospho) the addition of phosphatase inhibitors to the lysis buffer, and immediate light generation using chemiluminescent substrate.圖2. H1975細胞裂解物蛋白濃度之間的關系，細胞用含（磷酸）和不含（非磷酸）添加磷酸酶抑制劑的裂解緩沖液進行裂解，立即產生光化學發光底物。

Background

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

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表皮生長因子（EGF）受體是一個屬于HER/ErbB蛋白家族的跨膜酪氨酸激酶。配體的結合導致了受體的二聚化，自磷酸化，下游信號分子的激活，內化以及溶酶體降解 (1,2)。表皮生長因子受體（EGFR）在激酶域Tyr845位點的磷酸化與穩定激活回路有關，并且維持了酶的激活狀態，為底物蛋白提供了可結合的表位(3,4)。c-Src與 EGFR 在 Tyr845位點的磷酸化有關(5)。PLCγ的SH2結構域結合到磷酸化的Tyr992位點，將引起PLCγ介導的下游信號通路的激活(6)。EGFR?Tyr1045位點的磷酸化則為c-Cbl生成一個主要的?？课稽c，這個銜接蛋白將導致受體的泛素化以及EGFR活化后的降解(7,8)。GRB2銜接蛋白則結合到活化后的EGFR磷酸化的Tyr1068位點(9)。一對磷酸化EGFR殘基(Tyr1148 and Tyr1173)為Shc支架蛋白提供了?？奎c，這兩個位點都與MAPK激酶通路的激活有關(2)。EGFR在特定的絲氨酸和蘇氨酸殘基上的磷酸化減弱了EGFR激酶的活性。EGFR羧基末端殘基Ser1046 和 Ser1047被CaM 激酶II磷酸化，這兩個絲氨酸任何一個突變都將導致EGFR酪氨酸自身磷酸化的上調(10)。

7036 PathScan? Phospho-EGF Receptor (Tyr1068) Chemiluminescent Sandwich ELISA Kit

7074 Anti-rabbit IgG, HRP-linked Antibody

7187 PathScan? Phospho-EGF Receptor (Tyr1173) Sandwich ELISA Kit

7189 PathScan? Phospho-EGF Receptor (Tyr845) Sandwich ELISA Kit

7240 PathScan? Phospho-EGF Receptor (Tyr1068) Sandwich ELISA Kit

7250 PathScan? Total EGF Receptor Sandwich ELISA Kit

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9803 Cell Lysis Buffer (10X)