DESCRIPTION:

Recombinant Human β--\Nerve Growth Factor/β--\NGF produced in E. coli is a non--\glycosylated non--\covalent 
homodimer of two 118 amino acid polypeptides each with a molecular mass of 13.4 kD. Accession #: P01138
Synonyms :

Beta--\Nerve Growth Factor, Beta\NGF, NGF, NGFB
FORMULATION ：

Lyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.0
SHIPPING：

The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
STORAGE：
Lyophilized protein should be stored at < \20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4\7°C for 2\7 days.?
Aliquots of reconstituted samples are stable at < \20°C for 3 months.
RECONSTITUTION：

Always centrifuge tubes before opening.?Do not mix by vortex or pipetting. 
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in 1X PBS. 
Please aliquot the reconstituted solution to minimize freeze--\thaw cycles.

QUALITY CONTROL：

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Bioactivity*: ED50 is less than 1.0 ng/ml. Specific Activity is greater than 1 x 106?IU/mg. 

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*measuredbythedose--\dependent stimulationoftheproliferationofhumanTF--1 cells.SpecificActivityisg

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Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS--\PAGE. Endotoxin: Less than 0.1 ng/μg (1 IEU/μg). 
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