Superoxide Dismutase Activity Colorimetric Assay Kit
產(chǎn)品名稱： Superoxide Dismutase Activity Colorimetric Assay Kit
英文名稱： Superoxide Dismutase Activity Colorimetric Assay Kit
產(chǎn)品編號： ab65354
產(chǎn)品價格： null
產(chǎn)品產(chǎn)地： 英國
品牌商標(biāo)： abcam
更新時間： null
使用范圍：
- 聯(lián)系人 :
- 地址 : 上海市寶山區(qū)友誼路1506號東智廣場1109室
- 郵編 : 201900
- 所在區(qū)域 : 上海
- 電話 : 138****5983 點擊查看
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- 郵箱 : 342786402@qq.com
Overview
Product name
Superoxide Dismutase Activity Colorimetric Assay Kit
Product overview
The sensitive Superoxide Dismutase (SOD) Activity Assay Kit utilizes WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method. The lower limit for this kit is 0.1 U/ml of SOD activity.
Notes
Sample preparation.
1. Blood samples:
Collect blood using citrate or EDTA. Centrifuge at 1,000 x g for 10 min at 4°C. Transfer the plasma layer to a new tube without disturbing the puffy layer and store at -80°C until ready for analysis. Remove the puffy layer from the red cell pellet. Resuspend the erythrocytes in 5x volume of ice cold distilled water and centrifuge at 10,000 x g to pellet the erythrocyte membranes. Store the supernatant at -80°C until ready for analysis. Plasma can be diluted approx. 3-10x and the red cell lysate diluted approx. 100x prior to SOD assay.
2. Tissue and cell samples:
Tissue should be perfused with PBS or 150mM KCl to remove any red blood cells. Homogenize tissue or lyse cells in ice cold 0.1M Tris/HCl, pH 7.4 containing 0.5 % Triton X-100, 5mM ?-ME, 0.1mg/ml PMSF. Centrifuge the crude tissue homogenate/cell lysate at 14000 x g for 5 minutes at 4°C and discard the cell debris. The supernatant contains total SOD activity from cytosolic and mitochondria.
?Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at +4°C.
Components Identifier 100 tests SOD Assay Buffer WM 1 x 20ml SOD Dilution Buffer NM 1 x 10ml SOD Enzyme Solution Green 1 x 20μl WST Solution Red 1 x 1ml http://www.abcam.com/Superoxide-Dismutase-Activity-Colorimetric-Assay-Kit-ab65354.html