兔Anti-Goat IgG H&L (HRP)-抗體-抗體-生物在線
深圳市宇德立生物科技有限公司
兔Anti-Goat IgG H&L (HRP)

兔Anti-Goat IgG H&L (HRP)

商家詢價

產品名稱： 兔Anti-Goat IgG H&L (HRP)

英文名稱： 兔Anti-Goat IgG H&L (HRP)

產品編號： ab97100

產品價格： null

產品產地： 英國

品牌商標： abcam

更新時間： null

使用范圍：

深圳市宇德立生物科技有限公司
  • 聯系人 :
  • 地址 : 深圳市寶安區西鄉寶民二路賢基大廈4E
  • 郵編 :
  • 所在區域 : 廣東
  • 電話 : 133****4454 點擊查看
  • 傳真 : 點擊查看
  • 郵箱 : 1484332550@qq.com

?

  • 靶標種屬Goat
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • 經測試應用ICC,?IHC-P,?ELISA,?WBmore details
  • 偶聯物HRP

性能

  • 形式Liquid
  • 存放說明Shipped at 4°C. Store at +4°C.
  • 存儲溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • 濃度1 mg 濃度為 1 mg/ml?
  • 純度Immunogen affinity purified
  • 純化說明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共軛說明Molar enzyme/ antibody protein ratio is 4:1
  • 克隆多克隆
  • 同種型IgG
  • 常規說明Part of the?AbExcel?range.
  • 研究領域

應用

Our?Abpromise guarantee?covers the use of?ab97100?in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

應用 Abreviews 說明
ICC ? Use at an assay dependent concentration.
IHC-P ? 1/200 - 1/5000.
ELISA ? 1/10000 - 1/100000.
WB ? 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Rabbit Anti-Goat IgG H&L (HRP) 圖像

  • IHC image of?beta Actin?staining in?Human normal Colon?formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with?ab8229, 10μg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97100,?1/100 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes :
    Anti-beta Actin antibody (ab8229) at 1 μg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 μg per lane.??
    Secondary
    Lanes 1 - 2 :?Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/5000 dilution
    Lanes 3 - 4 :?Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/10000 dilution
    Lanes 5 - 6 :?Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time :?8 minutes