Target: Mouse

Host: Goat

Format: Whole IgG

Specificity: mouse IgG except IgM, Fcγ Fragment Specific

NoCross Reactivity: Human, Bovine, Rabbit Serum Proteins

Conjugate: Horseradish Peroxidase

Clonality: Polyclonal

Purity:Antigen specific purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Buffer:50% glycerol 0.01M PBS,pH 7.6

Stabilizer:14.5 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: None

Physical State: Liquid

Storage & Expiration Date: -20°C. for 24months

Suggested Working Concentration or Dilution Range:

1:500 -1:5,000 for immunohisto/cytochemistry.

1:5,000-1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

本產品基于間接ELISA實驗測試，排除與小鼠IgM外的其他小鼠IgG亞型的非Fab部分反應，與血清中非免疫球蛋白的其他組分沒有交叉反應；經過測試，本產品與人，牛和兔血清蛋白沒有任何交叉反應，不排除與其他物種的血清有交叉反應的可能（沒有測試其他物種數據）。

本二抗產品最顯著的用途是用于間接ELISA方法進行非IgM亞型單克隆抗體細胞株篩選：在單克隆抗體中如果篩選到IgM亞型的抗體非常棘手，本產品將IgM亞型單克隆抗體細胞株扼殺在搖籃中，基本原理是本二抗不會針對IgM亞型產生反應，因此篩選的時候得到的細胞株全部為非IgM亞型。