Anti-BRAF (V600M) Mouse Monoclonal Antibody-抗體-抗體-生物在線

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武漢紐斯特生物技術(shù)有限公司
Anti-BRAF (V600M) Mouse Monoclonal Antibody

Anti-BRAF (V600M) Mouse Monoclonal Antibody

商家詢價(jià)

產(chǎn)品名稱： Anti-BRAF (V600M) Mouse Monoclonal Antibody

英文名稱： Anti-BRAF (V600M) Mouse Monoclonal Antibody

產(chǎn)品編號(hào)： 26462

產(chǎn)品價(jià)格： null

產(chǎn)品產(chǎn)地： 美國(guó)

品牌商標(biāo)： NewEastBio

更新時(shí)間： null

使用范圍： ELISA、IF、IHC

武漢紐斯特生物技術(shù)有限公司
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BRAF (V600M) [26462]
[100ul]		 ?

Catalog Number:??26462
Gene Symbol:??BRAF, BRAF1, RAFB1
Description: ??Anti-BRAF (V600M) Mouse Monoclonal Antibody
Background: ??BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen: ??A synthetic peptide from the internal region of BRAF which includes the mutation of V600M, human origin.
Applications: ??ELISA, IF, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
IF: 1:50-1:100?
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes V600M mutant, but not wild-type BRAF of vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Immunofluorescence:??
Immunofluorescence of cells expressing BRAF proteins with anti-BRAF (V600M) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRAF (V600M) plasmid, pCDNA3-GFP-BRAF (WT) plasmid, pCDNA3-GFP-BRAF (V600A) plasmid, pCDNA3-GFP-BRAF (V600G) plasmid, pCDNA3-GFP-BRAF (V600K) plasmid or pCDNA3-GFP-BRAF (V600E) plasmid , then fixed and stained with anti-BRAF (V600M) monoclonal antibody (Cat. #26462).
Publications:
1.??Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
????Mod Pathol. 2013 Mar;26(3):414-20.
2.??Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
????Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
3.??Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
????human pathology. February 2015Volume 46, Issue 2, Pages 169–175
4.??Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
????human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
5.??BRAF V600E Mutation-Specific Antibody: A Review
????Seminars in Diagnostic Pathology

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