Active牛FGF basic全長蛋白質
產品名稱： Active牛FGF basic全長蛋白質
英文名稱： Basic fibroblast growth factor Basic fibroblast growth factor bFGF BFGF FGF 2 FGF B FGF-2 Fgf2 FGF2 basic FGF2_HUMAN FGFB Fibroblast growth
產品編號： ab129471
產品價格： 0
產品產地： 英國
品牌商標： abcam
更新時間： null
使用范圍： null
深圳市宇德立生物科技有限公司
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- 所在區域 : 廣東
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- 性質Recombinant
- 來源E. coli
- 氨基酸序列
- AccessionP03969
- 種屬Cow
- 序列The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.
- 分子量17 kDa
- 氨基酸1 to 155
技術指標
Our?Abpromise guarantee?covers the use of?ab129471?in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 生物活性The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be <0.1 ng/ml, corresponding to a specific activity of 10 MUnits/mg.
- 應用
HPLC
SDS-PAGE
Functional Studies
- 純度>= 97 % by SDS-PAGE.
ab129471 is purified by proprietary chromatographic techniques. Purity is greater than 97% as determined by HPLC and SDS-PAGE.
- 形式Lyophilised
- 補充說明Endotoxin Level: <0.1 ng/μg.
-
濃度由溶解方法決定?
制備和貯存
- 穩定性和存儲
Shipped at 4°C. Store at -20oC.
Constituent: 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 復溶Reconstitute in sterile water to a concentration not less than 0.1 mg/ml.
常規信息
-
別名
- Basic fibroblast growth factor
- Basic fibroblast growth factor bFGF
- BFGF
see all - 功能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
- 組織特異性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
- 序列相似性Belongs to the heparin-binding growth factors family.
- 翻譯后修飾Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. - 細胞定位Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.