Size??100ug
Cat code??200503
Species Cross-reactivity??H
Key Application??WB,IP
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??58kDa
Sensitivity??This antibody detects endogenous levels of Ikaros(C-terminus) and does not cross-react with related proteins.
Immunogen??Purified recombinant human Ikaros(C-terminus) protein fragments expressed in E.coli.
UniProt Number??Q13422
GeneBank ID??NM_006060
Subcellular location??Nucleus
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??IK1; LYF1; hIk-1; IKAROS; PRO0758; ZNFN1A1; Hs.54452
Gene_symbol??IK1; LYF1; hIk-1; IKAROS; PRO0758; ZNFN1A1; Hs.54452
Summary??Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher
affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the
enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene
during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and
HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex
(PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells.
Confers early temporal competence to retinal progenitor cells (RPCs).
UniPort summary  Function??Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity)
Application Images :??
Western blot detection of Ikaros(C-terminus) antibody in Ramos&Jurkat cell lysates using Ikaros(C-terminus) antibody (1:1000 diluted).
Predicted band size: 58KDa
Observed band size: 58KDa.
Immunoprecipitation analysis of Hela cell lysate using IKZF1 antibody.