活性ErbB 2 蛋白
產(chǎn)品名稱： 活性ErbB 2 蛋白
英文名稱： Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog C erb B2/neu protein CD340 CD340 antig
產(chǎn)品編號： ab60866
產(chǎn)品價格： 0
產(chǎn)品產(chǎn)地： 英國
品牌商標： abcam
更新時間： null
使用范圍： null
- 聯(lián)系人 :
- 地址 : 深圳市寶安區(qū)西鄉(xiāng)寶民二路賢基大廈4E
- 郵編 :
- 所在區(qū)域 : 廣東
- 電話 : 133****4454 點擊查看
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- 郵箱 : 1484332550@qq.com
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- 性質(zhì)Recombinant
- 來源Insect cells
技術(shù)指標
Our?Abpromise guarantee?covers the use of?ab60866?in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 應用
SDS-PAGE
Functional Studies
- 純度> 90 % by SDS-PAGE.
Purity: >90% as determined by densitometry.
- 形式Liquid
- 補充說明
ab204877?(Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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濃度10 μg 濃度為 0.1 mg/ml?100 μg 濃度為 0.1 mg/ml?
制備和貯存
- 穩(wěn)定性和存儲
Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5This product is an active protein and may elicit a biological response in vivo, handle with caution.
常規(guī)信息
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別名
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
- C erb B2/neu protein
- CD340
see all - 功能Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5-TCAAATTC-3 sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. - 組織特異性Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
- 疾病相關(guān)Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. - 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. - 翻譯后修飾Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Signaling via SEMA4C promotes phosphorylation at Tyr-1248.
- 細胞定位Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
- Information by UniProt
Active ErbB 2 protein 圖像
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Functional Studies - ErbB 2 protein (Cytoplasmic domain) (ab60866)Sample Kinase Activity Plot. -
SDS-PAGE - ErbB 2 protein (Cytoplasmic domain) (ab60866)ab60866 on SDS-PAGE, MW ~116 kDa.