BNIP3 Rabbit Polyclonal Antibody
產(chǎn)品名稱: BNIP3 Rabbit Polyclonal Antibody
英文名稱: BNIP3 Rabbit Polyclonal Antibody
產(chǎn)品編號(hào): K6330
產(chǎn)品價(jià)格: null
產(chǎn)品產(chǎn)地: 上海
品牌商標(biāo): 雅吉生物
更新時(shí)間: null
使用范圍: WB, IF, ICC
- 聯(lián)系人 :
- 地址 : 上海市閔行區(qū)元江路5500號(hào)第1幢5658室
- 郵編 :
- 所在區(qū)域 : 上海
- 電話 : 158****3937 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : yajikit@163.com
| 來(lái)源 | 用途 | 交叉反應(yīng)性 | 理論分子量 | 實(shí)際分子量 |
| Rabbit | WB, IF, IHC | H, M, R | 27kDa | 22-70kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液,可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整):
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | - | 1:50-1:200 | 1:50-1:200 | 1:50-1:200 | - | - | - |
抗體詳細(xì)信息如下::
| About this Antibody | |
| Name | BNIP3 Rabbit Polyclonal Antibody |
| Category | Rabbit Polyclonal Antibody (pAb); Primary antibody |
| Isotype | IgG |
| Purification method | Affinity purification |
| Positive samples | MCF7, NCI-H460, Jurkat, SH-SY5Y, Mouse kidney, Mouse heart, Mouse liver, Rat brain |
| Cellular location | Mitochondrion, Mitochondrion outer membrane, Single-pass membrane protein |
| Customer validation | WB (Human); IHC (Mouse) |
| About the Immunogen | |
| Immunogen | Recombinant fusion protein of human BNIP3 (NP_004043.3). |
| Sequence | MGDAAADPPGPALPCEFLRPGCGAPLSPGAQLGRGAPTSAFPPPAAEAHPAARRGLRSPQLPSGAMSQNGAPGMQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDD |
| Gene ID | 664 |
| Swiss Prot | Q12983 |
| Synonyms | BNIP3; NIP3; BCL2 interacting protein 3 |
| Category | Regulation of Apoptosis; Inhibition of Apoptosis; Autophagy Signaling; Mitochondrial Control of Apoptosis |
| Background | This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. |
包裝清單:
| 產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 包裝 |
| AF6330 | BNIP3 Rabbit Polyclonal Antibody | 50μl |
| AZ050 | Western一抗稀釋液 | 50ml |
| — | 說(shuō)明書 | 1份 |
保存條件:
BNIP3 Rabbit Polyclonal Antibody -20oC保存,Western一抗稀釋液-20oC或4oC保存,一年有效。Western一抗稀釋液優(yōu)先推薦4oC保存,長(zhǎng)期不使用可以考慮-20oC保存,但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn),請(qǐng)注意回收使用過(guò)的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體,包括已經(jīng)使用過(guò)的稀釋抗體,請(qǐng)4℃保存。
回收后重復(fù)使用的抗體,使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象,可以10,000g離心1-3分鐘,取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況,則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得最佳的檢測(cè)效果,請(qǐng)考慮使用上述檢測(cè)專用的一抗稀釋液。
本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
代表性圖片:
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| Fig. 1. Western blot analysis of extracts of various cell lines, using BNIP3 antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: QuickBlock? Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s. | Fig. 2. Immunohistochemistry of paraffin-embedded mouse kidney using BNIP3 antibody at dilution of 1:100 (40x lens). |
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| Fig. 3. Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody at dilution of 1:100 (40x lens). | Fig. 4. Immunofluorescence analysis of HeLa cells using BNIP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining. |




