"抗體，環指蛋白149抗體
產品貨號：YM-S10183-X
規 格：0.1ml/0.2ml
濃 度：1mg/1ml.
抗體來源：Rabbit / mouse
克隆類型：兔來源為polyclonal，鼠來源為monoclonal
產品類型：一抗 單抗
性 狀：凍干粉或液體
亞 型：IgG
純化方法：affinity purified by Protein A
儲 存 液：0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
抗體，環指蛋白149抗體Rules:
（1） the initial reaction to produce antibodies to antigens: when thefirst to enter the body, need to produce antibodies after certainincubation period can, and antibody production quantity are not many, inthe body to maintain the time is short.
（2） once again: when the same antigen antibody reaction to producesecond times into the body, at the beginning, as part of the originalantibody binding and once again into the antigen antibody, can make theoriginal amount reduced slightly. Subsequently, rapid increase antibodytiter, several times more than the initial reaction can be generated toa few times, retained in the body of a lot of time.
（3） recall response to produce antibodies: antibodies produced byantigen stimulation of the body, after a certain period of time candisappear gradually. If the re exposure to antigen, the antibody hasfaded fast rise. As the body with the same antigen again first, thenknown as the specific recall responses; and if the initial reactions ofdifferent, it is known as a nonspecific recall response. Increasednonspecific memories to elicit antibody responses are temporary, shorttime soon fall.
抗體產品WB實驗中的對照系統：
Western blotting實驗原理：免疫印跡(immunoblotting)又稱蛋白質印跡(Western blotting)，它是根據抗原抗體的特異性結合檢測復雜樣品中的某種蛋白的方法。該法是在凝膠電泳和固相免疫測定技術基礎上發展起來的一種新的免疫生 化技術。由于免疫印跡具有SDS-PAGE的高分辨力和固相免疫測定的高特異性和敏感性，現已成為蛋白分析的一種常規技術。免疫印跡常用于鑒定某種蛋白， 并能對蛋白進行定性和半定量分析。
WB對照系統：陽性對照：最好有標準品，或陽性血清、陽性上清。
陰性對照：測血時用相應小鼠未免疫血清，測培養上清，用無克隆培養上清?？瞻讓φ眨翰患右豢?，用88%BSA代替。無關對照（替代對照）：用無關項目一抗，或無關項目的抗體。
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