The NEBNext Ultra II FS DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step. The fast, user-friendly workflow includes reliable enzymatic DNA fragmentation, requires minimal hands-on time, resulting in excellent library yields and GC coverage without requiring PCR. 

Advantages:
Enjoy flexible input amounts from 50 ng to 500 ng of intact DNA.
Experience reliable fragmentation with a single protocol, regardless of DNA input amount or GC content.
Perform fragmentation, end repair and dA-tailing with a single enzyme mix.
Get more of what you need with reliably high library yields.
Prepare PCR-free libraries with a broad range of sample types.
Save time with streamlined workflows, reduced hands-on time, and automation compatibility.
Use with the NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395) or other Illumina-compatible index adaptors with a 3’ single T overhang.