詳細(xì)配制內(nèi)容及參數(shù)資料

1.1 特點(diǎn)

l可以做更準(zhǔn)確的藥代動(dòng)力學(xué)和毒物學(xué)的研究

l更清楚準(zhǔn)確的反應(yīng)了體內(nèi)血腦屏障的特性

l模擬重要的內(nèi)皮細(xì)胞與星形膠質(zhì)細(xì)胞之間的作用

l用電力方法檢測了血腦屏障的完整性

l可以用真實(shí)的細(xì)胞做實(shí)驗(yàn)

l比現(xiàn)存的靜態(tài)模型更能形成緊密的連接

l降低藥物研發(fā)的成本

1.2 配置及參數(shù)資料

TEER檢測系統(tǒng)CARTRIDGE底板

4個(gè)DIV-BBB盒子檢測軟件電纜線

DIV-BBB?管腔

經(jīng)毛細(xì)管孔徑：0.2um

中空纖維數(shù)量：19

腔內(nèi)總體積：0.0123in³= 0.202cm³

腔內(nèi)表面積：2.09 in³= 13.5cm²

腔外體積：0.070 in³= 1.15cm³

腔外中空纖維總面積：3.50 in³= 22.6cm²

TEER檢測系統(tǒng)

TEER檢測系統(tǒng)為研發(fā)人員提供了一個(gè)快速、簡便地評(píng)估血腦屏障完整性方法，此外，電阻抗值（＞1000 Ω-cm²）也非常接近體內(nèi)數(shù)值。TEER檢測系統(tǒng)同時(shí)配帶了所需檢測軟件、電線和襯板，可供4個(gè)DIV-BBB管腔同時(shí)使用。其中檢測軟件可以自定義設(shè)置泵的流速、頻率、波形等參數(shù)。其它特性及優(yōu)點(diǎn)見下表：

脈動(dòng)泵

脈搏率和作用周期完全模擬體內(nèi)血液動(dòng)力學(xué)設(shè)計(jì)，貼近體內(nèi)環(huán)境，試驗(yàn)準(zhǔn)確，脈搏率可設(shè)置在30-120/分范圍內(nèi)。其特性和優(yōu)點(diǎn)見下：

電腦配置最低要求

?IBM兼容電腦，400MHz或者更快

?Window 2000或者更高系統(tǒng)

?128MB存儲(chǔ)器（推薦256MB）

?

About Flocel

Every year millions of dollars are spent by pharmaceutical companies to develop and test drugs using in vitro models of the Blood-Brain Barrier (BBB). Many of these cases end up not working in vivo.CleveMedhas licensed technology fromThe Cleveland Clinic Foundation and formed Flocel Inc, to manufacture a practical in vitro model that more accurately reflects the BBB characteristics. Flocel's dynamic in vitro blood-brain barrier (DIV-BBB) technology helps researchers in the United States and Europe conduct in vitro studies.

Flocel also offers atesting service for BBB permeability compounds. To learn more about our testing services, clickhere.

Introduction to the Blood-Brain Barrier

TThe blood-brain barrier (BBB) explains why certain substances circulating in the blood are able to get into the brain and others do not. The BBB is formed by brain capillaries that look like simple tubes of endothelial cells. Experimental and clinical evidence suggests that the BBB maintains the chemical environment for neuronal function and protects the brain from harmful substances. The blood-brain barrier is therefore crucial for complex issues such as drug delivery, pathogenesis of chronic neurological diseases, and issues related to bio-defense. We have developed a practical in vitro model that accurately reflects BBB characteristics.

Benefits of Flocel’s in vitro BBB Model

In contrast to other available models of the blood-brain barrier, Flocel's Dynamic In vitro Blood-Brain Barrier (DIV-BBB) respects the anatomical aspects of the in situ endothelial cell-astrocyte interactions and replicates the physiological levels of shear stress to which in situ endothelial cells are exposed. The DIV-BBB allows formation of physiological transendothelial resistance, and formation of gap junctions that can be easily visualized at the electron microscope or determined experimentally with the use of tracers.

Mission

Flocel Inc. is a biotechnology company dedicated to innovation towards helping the research community better conduct and advance in vitro studies.

In vitro BBB Technology

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